1. Linkage, Recombination and Eukaryotic Mapping Part 2

2. Outline Gene mapping in Humans –Pedigrees and linkage Physical Chromosome mapping

3. Nail-Patella Syndrome –Autosomal dominant: abnormal fingernails and absent (underdeveloped kneecaps) –ABO blood type alleles linkage

4. 2/13= 0.154 recombination frequency (2 out of 13 offspring)

5. Physical Chromosome Mapping Provides us alternative methods for gene mapping that do not rely on rates of crossing over to determine gene location

6. Methods that are considered “physical mapping of the chromosome” Deletion mapping Somatic Cell hybridization In Situ Hybridization DNA Sequencing

7. DELETION MAPPING (this has been used to map Muscular Dystrophy gene to X chromosome 50% have mutation all have normal phenotype

8. Deletion Mapping Can also be done with homozygous recessive Homozygous wt+ but with deletion(s) Deletion area abab abab B+ Offspring will be: aB+ We now know this deletion includes the locus that is within the region deleted for A+ but not for B+ Crossed with abab

9. f?a f? c e d b Mutations del a b c d e f 1m+m++m 2m+++++ 3+mmmm+ 4++mmm+ 5+++mm+ 6+m+m++ You can perform this a panel of deletions. ac d etc.

10. SOMATIC CELL HYBRIDIZATION + Human somatic cell

11. You set up a panel of these hybrid cells that include a random selection of human chromosomes Look for which cell lines produce the product AND which chromosomes are present. Bingo! You can determine which gene is associated with which chromosome!

12. In Situ Hybridization detects genes on chromsomes

13. DNA SEQUENCING More to come with our molecular discussions

14. Practice Physical Mapping Questions 4, 5, 26 and 27 at the back of chapter 7 to try out your skills at deletion analysis and somatic-cell hybridization. I will post the answer for question 26 (others are in the book). Does not have to be handed in—just to practice to see if it all makes sense.

15. The End