1. DNA STRUCTURE

2. NUCLEIC ACIDS Nucleic acids are polymers Nucleic acids are polymers Monomer---nucleotides Monomer---nucleotides Nitrogenous bases Nitrogenous bases Purines Purines Pyrimidines Pyrimidines Sugar Sugar Ribose Ribose Deoxyribose Deoxyribose Phosphates Phosphates +nucleoside=nucleotide +nucleoside=nucleotide } Nucleosides

3. The Sugars

4. The Bases PURINES PYRIMIDINES

5. Bases of DNA (and RNA) RNA only DNA only Purines: Pyrimidines:

6. Nucleotides and Nucleosides

7. Chemical Structure of DNA and RNA Figure 4.1 RNA DNA Nucleotide Nucleoside 1’ 2’ 4’ The C is named 1’-5’ Resume

8. Nucleotides and Nucleosides BASENUCLEOSIDEDEOXYNUCLEOSIDE AdenineAdenosine2-deoxyadenosine GuanineGuanosine2-deoxyguanosine CytosineCytodine2-deoxycytodine UracilUridine Not usually found Thymine 2-deoxythymidine Nucleotides are nucleosides + phosphate

9. Nucleotide Analogs as Drugs

10. make up 13-34% of the dry weight in bacteria deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) O CC CC Base H H or OH HH H H CH 2 OP OH O HO Nucleotide: a building block Sugar: RNA – ribose (OH) DNA – deoxyribose (H) Bases: adenine (A), cytosine (C), guanine (G), thymine (T) RNA uses uracil (U) instead of thymine Nucleoside: base + sugar certain nucleotides serve as a storage of energy and reducing power e.g.ATP -> ADP -> AMP hydrolysis (energy is released) Nucleic Acids

11. DNA Stabilization– Complementary Base Pairing

12. DNA Stabilization-Base Stacking

13. DNA Stabilization--H-bonding between DNA base pair stacks

14. Advantages to Double Helix Stability---protects bases from attack by H 2 O soluble compounds and H 2 O itself. Stability---protects bases from attack by H 2 O soluble compounds and H 2 O itself. Provides easy mechanism for replication Provides easy mechanism for replication

15. Physical Structure (cont’d) Chains are anti-parallel (i.e in opposite directions) Chains are anti-parallel (i.e in opposite directions) Diameter and periodicity are consistent Diameter and periodicity are consistent 2.0 nm 2.0 nm 10 bases/ turn 10 bases/ turn 3.4 nm/ turn 3.4 nm/ turn Width consistent because of pyrimidine/purine pairing Width consistent because of pyrimidine/purine pairing

16. Physical Structure (cont’d)

17. G-C Content A=T, G=C, but AT ≠GC A=T, G=C, but AT ≠GC Generally GC~50%, but extremely variable Generally GC~50%, but extremely variable EX. EX. Slime mold~22% Slime mold~22% Mycobacterium~73% Mycobacterium~73% Distribution of GC is not uniform in genomes Distribution of GC is not uniform in genomes

18. CONSEQUENCES OF GC CONTENT GC slightly denser GC slightly denser  Higher GC DNA moves further in a gradient  Higher GC DNA moves further in a gradient Higher # of base pairs=more stable DNA, i.e. the strands don’t separate as easily. Higher # of base pairs=more stable DNA, i.e. the strands don’t separate as easily.

19. FORMS OF DNA

20. Supercoiling

21. Cruciform Structures Another adaptation to supercoiling Associated with palindromes

22. DNA is Dynamic Like proteins, DNA has 3 º structure Like proteins, DNA has 3 º structure Why so many deviations from normal conformation? Why so many deviations from normal conformation? Effects on transcription (gene expression) Effects on transcription (gene expression) Enhances responsiveness Enhances responsiveness May also serve in packaging May also serve in packaging NOTE: most cellular DNA exists as protein containing supercoils NOTE: most cellular DNA exists as protein containing supercoils

23. Denaturation of DNA Denaturation by heating. Denaturation by heating. How observed? How observed? A 260 A 260 For dsDNA, For dsDNA, A 260 =1.0 for 50 µg/ml A 260 =1.0 for 50 µg/ml For ssDNA and RNA A 260 =1.0 for 38 µg/ml For ssDNA and RNA A 260 =1.0 for 38 µg/ml For ss oligos For ss oligos A 260 =1.0 for 33 µg/ml A 260 =1.0 for 33 µg/ml Hyperchromic shift Hyperchromic shift The T at which ½ the DNA sample is denatured is called the melting temperature (T m )

24. Importance of T m Critical importance in any technique that relies on complementary base pairing Critical importance in any technique that relies on complementary base pairing Designing PCR primers Designing PCR primers Southern blots Southern blots Northern blots Northern blots Colony hybridization Colony hybridization

25. Factors Affecting T m G-C content of sample G-C content of sample Presence of intercalating agents (anything that disrupts H-bonds or base stacking) Presence of intercalating agents (anything that disrupts H-bonds or base stacking) Salt concentration Salt concentration pH pH Length Length

26. Renaturation Strands can be induced to renature (anneal) under proper conditions. Factors to consider: Strands can be induced to renature (anneal) under proper conditions. Factors to consider: Temperature Temperature Salt concentration Salt concentration DNA concentration DNA concentration Time Time

27. C o t Curves

28. What Do C o t Curves Reveal? Complexity of DNA sample Complexity of DNA sample Reveals important info about the physical structure of DNA Reveals important info about the physical structure of DNA Can be used to determine T m for techniques that complementary base pairing. Can be used to determine T m for techniques that complementary base pairing.

29. Complexity of DNA- Factors Repetitive Sequences Single Copy Genes Single Copy Genes Highly repetitive (hundreds to millions) Highly repetitive (hundreds to millions) Randomly dispersed or in tandem repeats Randomly dispersed or in tandem repeats Satellite DNA Satellite DNA Microsatellite repeats Microsatellite repeats Miniisatellite repeats Miniisatellite repeats Middle repetitive (10- hundreds) Middle repetitive (10- hundreds) Clustered Clustered Dispersed Dispersed Slightly repetitive (2-10 copies) Slightly repetitive (2-10 copies)

30. Highly repetitive sequences Middle repetitive Middle repetitive sequences Unique sequences Renaturation curves of E. coli and calf DNA

31. RNA Types Types mRNA mRNA tRNA tRNA rRNA rRNA It’s still an RNA world It’s still an RNA world snRNA snRNA siRNA siRNA Ribozymes Ribozymes

32. Behavior in Acids Dilute or mild acidic conditions Dilute or mild acidic conditions Intermediate conditions. EX. 1N HCl @ 100ºC for 15m : Depurination Intermediate conditions. EX. 1N HCl @ 100ºC for 15m : Depurination Harsher treatment-EX. 2-6N HCl, higher temps: Depyrimidination. Harsher treatment-EX. 2-6N HCl, higher temps: Depyrimidination. NOTE: some phosphodiester bond cleavage observed during depurination, much more during depyrimidination NOTE: some phosphodiester bond cleavage observed during depurination, much more during depyrimidination

33. Behavior in Bases N-glycosidic bonds stable in mild alkaline conditions N-glycosidic bonds stable in mild alkaline conditions DNA melts DNA melts Phosphodiester linkages in DNA and RNA show very different behavior in weak bases (EX 0.3 N KOH @37 º C ~1 hr.) Phosphodiester linkages in DNA and RNA show very different behavior in weak bases (EX 0.3 N KOH @37 º C ~1 hr.) Phosphodiester

34. RNA Hydrolysis in Alkaline Solutions 2,3 cyclic nucleotide

35. Hydrolysis by Enzymes Nuclease—catalyzes hydrolysis of phosphodiester backbone Nuclease—catalyzes hydrolysis of phosphodiester backbone Exonucleases Exonucleases Endonucleases Endonucleases General. Ex DNAse I General. Ex DNAse I Specific Ex. Restriction endonucleases Specific Ex. Restriction endonucleases Ribozymes Ribozymes

36. Restriction Enzymes

37. RIBOZYMES Catalytic RNA Catalytic RNA Can work alone or with proteins Can work alone or with proteins Therapeutic applications? Therapeutic applications?

38. SEQUENCING Purpose—determine nucleotide sequence of DNA Purpose—determine nucleotide sequence of DNA Two main methods Two main methods Maxam & Gilbert, using chemical sequencing Sanger, using dideoxynucleotides

39. The Sanger Technique Uses dideoxynucleotides (dideoxyadenine, dideoxyguanine, etc) Uses dideoxynucleotides (dideoxyadenine, dideoxyguanine, etc) These are molecules that resemble normal nucleotides but lack the normal -OH group. These are molecules that resemble normal nucleotides but lack the normal -OH group.

40. Because they lack the -OH (which allows nucleotides to join a growing DNA strand), replication stops. Because they lack the -OH (which allows nucleotides to join a growing DNA strand), replication stops. Normally, this would be where another phosphate Is attached, but with no - OH group, a bond can not form and replication stops

41. The Sanger Method Requires Multiple copies of single stranded template DNA Multiple copies of single stranded template DNA A suitable primer (a small piece of DNA that can pair with the template DNA to act as a starting point for replication) A suitable primer (a small piece of DNA that can pair with the template DNA to act as a starting point for replication) DNA polymerase (an enzyme that copies DNA, adding new nucleotides to the 3’ end of the template DNA polymerase (an enzyme that copies DNA, adding new nucleotides to the 3’ end of the template A ‘pool’ of normal nucleotides A ‘pool’ of normal nucleotides A small proportion of dideoxynucleotides labeled in some way ( radioactively or with fluorescent dyes) A small proportion of dideoxynucleotides labeled in some way ( radioactively or with fluorescent dyes)

42. The template DNA pieces are replicated, incorporating normal nucleotides, but occasionally and at random dideoxy (DD) nucleotides are taken up. The template DNA pieces are replicated, incorporating normal nucleotides, but occasionally and at random dideoxy (DD) nucleotides are taken up. This stops replication on that piece of DNA This stops replication on that piece of DNA The result is a mix of DNA lengths, each ending with a particular labeled DDnucleotide. The result is a mix of DNA lengths, each ending with a particular labeled DDnucleotide. Because the different lengths ‘travel’ at different rates during electrophoresis, their order can be determined. Because the different lengths ‘travel’ at different rates during electrophoresis, their order can be determined.