1. About Science Prof OnlineScience Prof Online PowerPoint Resources Science Prof Online (SPO) is a free science education website that provides fully-developed Virtual Science Classrooms, science-related PowerPoints, articles and images. The site is designed to be a helpful resource for students, educators, and anyone interested in learning about science. The SPO Virtual Classrooms offer many educational resources, including practice test questions, review questions, lecture PowerPoints, video tutorials, sample assignments and course syllabi. New materials are continually being developed, so check back frequently, or follow us on Facebook (Science Prof Online) or Twitter (ScienceProfSPO) for updates. Many SPO PowerPoints are available in a variety of formats, such as fully editable PowerPoint files, as well as uneditable versions in smaller file sizes, such as PowerPoint Shows and Portable Document Format (.pdf), for ease of printing. Images used on this resource, and on the SPO website are, wherever possible, credited and linked to their source. Any words underlined and appearing in blue are links that can be clicked on for more information. PowerPoints must be viewed in slide show mode to use the hyperlinks directly. Several helpful links to fun and interactive learning tools are included throughout the PPT and on the Smart Links slide, near the end of each presentation. You must be in slide show mode to utilize hyperlinks and animations. This digital resource is licensed under Creative Commons Attribution-ShareAlike 3.0: http://creativecommons.org/licenses/by-sa/3.0/ Alicia Cepaitis, MS Chief Creative Nerd Science Prof Online Online Education Resources, LLC alicia@scienceprofonline.com From the Virtual Cell Biology Classroom on ScienceProfOnline.comVirtual Cell Biology ClassroomScienceProfOnline.com Image: Compound microscope objectives, T. Port Tami Port, MS Creator of Science Prof Online Chief Executive Nerd Science Prof Online Online Education Resources, LLC info@scienceprofonline.com

2. Molecular Genetics Transcription & Translation Image: Transcription, National Human Genome Research InstituteTranscription From the Virtual Cell Biology Classroom on ScienceProfOnline.comVirtual Cell Biology ClassroomScienceProfOnline.com

3. Genetics Terminology ________ - Complete complement of an organism’s DNA. DNA Cellular DNA is organized in _____________. ______ have specific places on chromosomes. Q: What does the term expressed have to do with today’s lecture? Image: Chromosome & gene, Graham Colm, National Human Genome Research InstituteChromosome & gene From the Virtual Cell Biology Classroom on ScienceProfOnline.comVirtual Cell Biology ClassroomScienceProfOnline.com

4. Nucleic Acids: DNA Structure Image: DNA Detail Diagram: Madprime; DNA Molecule, Biology CornerDNA Detail Diagram: DNA Molecule From the Virtual Cell Biology Classroom on ScienceProfOnline.comVirtual Cell Biology ClassroomScienceProfOnline.com

5. Nucleic Acids – RNA Structure RNARNA is typically a single- stranded molecule. Purine Bases (double ring) Adenine & Guanine Pyrimidine Bases (single ring) Cytosine & _______ Image: RNA, Biology CornerRNA From the Virtual Cell Biology Classroom on ScienceProfOnline.comVirtual Cell Biology ClassroomScienceProfOnline.com

6. Nucleic Acid Structure Image: Comparison of DNA & RNA, Wiki CommonsComparison of DNA & RNA From the Virtual Cell Biology Classroom on ScienceProfOnline.comVirtual Cell Biology ClassroomScienceProfOnline.com See SPO Class Notes article on Nucleotides & Nucleic Acids.Nucleotides & Nucleic Acids

7. Nucleic Acid Function Transcription Image: Transcription, National Human Genome Research Institute.Transcription From the Virtual Cell Biology Classroom on ScienceProfOnline.comVirtual Cell Biology ClassroomScienceProfOnline.com

8. Replication & Transcription MAKING DNA = ______________ Making a copy of the genetic material. Think “duplication.” Image: Replication Diagram: Madprime, Wiki; Transcription, National Human Genome Research InstituteReplication DiagramTranscription MAKING RNA= ___________ Transferring the genetic code information (DNA) into RNA.DNARNA Think of a medical transcriptionist copying the physicians words into another format. From the Virtual Cell Biology Classroom on ScienceProfOnline.comVirtual Cell Biology ClassroomScienceProfOnline.com

9. Transcription Process by which a DNA sequence is copied to produce a complementary RNA. In other words, it is the transfer of genetic information from DNA into RNA. Like replication, but making RNA.replication Beginning of the process that ultimately leads to the translation of the genetic code (via mRNA) into a protein.protein Image: Transcription, National Human Genome Research Institute.Transcription From the Virtual Cell Biology Classroom on ScienceProfOnline.comVirtual Cell Biology ClassroomScienceProfOnline.com

10. Types of RNA Images: Blueprint, clipart; Factory, Andreas Praefcke; Truck, PRA.FactoryTruck Genetic information copied from DNA is transferred to 3 types of RNA:RNA __________ RNA: mRNA Copy of information in DNA that is brought to the ribosome and translated into protein by tRNA & rRNA.protein __________ RNA: rRNA Most of the RNA in cells is associated with structures known as ribosomes, the protein factories of the cells. It is the site of translation where genetic information brought by mRNA is translated into actual proteins. ___________ RNA: tRNA Brings the amino acid to the ribosome that mRNA coded for. From the Virtual Cell Biology Classroom on ScienceProfOnline.comVirtual Cell Biology ClassroomScienceProfOnline.com

11. Nucleic Acid Function Translation Image: Peptide Synthesis Diagram: Boumphreyfr, WikiPeptide Synthesis Diagram From the Virtual Cell Biology Classroom on ScienceProfOnline.comVirtual Cell Biology ClassroomScienceProfOnline.com

12. Replication, Transcription, Translation MAKING DNA Making a copy of the genetic material = Replication When you think “replication” think “duplication” Where does replication occur in prokaryotes? Eukaryotes?prokaryotesEukaryotes MAKING RNA Transferring genetic code (DNA) to RNA = TranscriptionTranscription Think of a medical transcriptionist copying the physicians words into another format. Where does transcription occur in prokaryotes? Eukaryotes? MAKING PROTEINS Making proteins = ______________. Think of how translation relates to languages. The translation of biology translates DNA information into proteins.proteins Images: Replication Diagram: Madprime; Peptide Synthesis Diagram: Boumphreyfr, WikiReplication DiagramPeptide Synthesis Diagram From the Virtual Cell Biology Classroom on ScienceProfOnline.comVirtual Cell Biology ClassroomScienceProfOnline.com

13. TranslationTranslation & The ________ _______ _____________ (which contain rRNA) make proteins from the messages encoded in mRNA.proteins The genetic instructions for a polypeptide chain are ‘written’ in the DNA as a series of 3-nucleotide ‘words.’ ______ on mRNA ___________ on tRNA ‘U’ (uracil) replaces ‘T’ in RNA This is the genetic code. Q: Where does translation occur in prokaryotes?prokaryotes Q: Eukaryotes?Eukaryotes Image: Codon-Anticodon pairing, Yikrazuul, Wiki; Peptide Synthesis Diagram: Boumphreyfr, Wiki; Ribosome translating protein, Xvazquez.Codon-Anticodon pairingPeptide Synthesis DiagramRibosome translating protein Anti-codon Codon From the Virtual Cell Biology Classroom on ScienceProfOnline.comVirtual Cell Biology ClassroomScienceProfOnline.com Click here Click here for animation of ribosome building a protein.

14. Overview ______________ Synthesis of RNA under the direction of DNA (mRNA). ______________ Actual synthesis of a polypeptide (protein) under the direction of mRNA. Eukaryotic Cell Diagram: Transcription & Translation, NIHTranscription & Translation From the Virtual Cell Biology Classroom on ScienceProfOnline.comVirtual Cell Biology ClassroomScienceProfOnline.com

15. Overview See SPO Class Notes article on DNA Function: Transcription & Translation.DNA Function: Transcription & Translation

16. Overview

17. Confused? Here are links to fun resources that further explain genetic transcription & translation: Molecular Genetics: Transcription & Translation Main Page on the Virtual Cell Biology Classroom of Science Prof Online.Transcription & Translation Science Prof Online “That Spells DNA” song by Jonathan Coulton.“That Spells DNA” DNA Transcription step-through animation by John Kyrk.DNA Transcription Transcribe & Translate a Gene, from University of Utah.Transcribe & Translate a Gene DNA Transcription and Protein Assembly animated movie by RedAndBrownPaperBag.DNA Transcription and Protein Assembly Genetic by Sonic Youth.Genetic Transcription and Translation animated movie from PBS production “DNA: The Secret of Life.Transcription and Translation “ The Protein Synthesis Song ” by MrsPurpleMonster18. The Protein Synthesis Song (You must be in PPT slideshow view to click on links.) From the Virtual Cell Biology Classroom on ScienceProfOnline.comVirtual Cell Biology ClassroomScienceProfOnline.com

18. In-class Assignment At the end of some lectures, I will give you some type of in-class assignment or homework to evaluate your understanding of today’s topic. This assignment will always be open-book. Today, if assigned you will be completing an activity on the topic of DNA Transcription & Translation. See the ScienceProfOnline Virtual Cell Molecular Genetics: DNA Transcription & Translation Lecture for a printable Word.doc of this assignment.ScienceProfOnline From the Virtual Cell Biology Classroom on ScienceProfOnline.comVirtual Cell Biology ClassroomScienceProfOnline.com

19. Are you feeling blinded by science ? Do yourself a favor. Use the… Virtual Cell Biology Classroom (VCBC) ! The VCBC is full of resources to help you succeed, including:VCBC practice test questions review questions study guides and learning objectives PowerPoints on other topics Access the Virtual Cell Biology Classroom (VCBC) on the Science Prof Online website www.ScienceProfOnline.comVirtual Cell Biology Classroom www.ScienceProfOnline.com Images: Blinded With Science album, Thomas Dolby; Endomembrane system, Mariana Ruiz, WikiBlinded With ScienceEndomembrane system