1. Analysis of plankton by flow cytometry

8. Sampling Fresh sample Unstained samples

10. Fixation Sampling Fresh sample Unstained samples

11. Paraformaldehyde fixation

13. Sampling Fresh sampleFixation Unstained samples Freezing -80°C Thawing Stained samples

14. Fluorescent dyes used for the analysis of nucleic acids of marine samples BO-PRO-1 PO-PRO-1 TO-PRO-1 YO-PRO-1 BOBO-1 POPO-1 TOTO-1 YOYO-1 SYTOSYTO SYTOX Green PicoGreen SYBR Green-I Acridine Orange Propidium iodide Ethidium bromide Chromomycin A3 Mythramycin DAPI Hoechst 33342 Hoechst 33258 SYBR Green-II OliGreen RiboGreen

15. Bacterial Staining: SYBR Green-I

16. Addition of beads Sampling Fresh sampleFixation Freezing -80°C Thawing Stained samples Unstained samples

18. Sampling Fresh sampleFixation Freezing -80°C Thawing Stained samples Addition of beads Unstained samples CytoWin Cell parameters

21. Vaulot and Marie, 1999 JGR Equatorial Pacific

22. Vaulot and Marie, 1999 JGR Equatorial Pacific

23. McycleSampling Fresh sampleFixation Freezing -80°C Thawing Stained samples Addition of beads CytoWin Cell parameters Unstained samples Cell cycle analysis

25. Vaulot et al., 1995 Science

28. Depth profile Almofront2 CTD512

29. DNA QUANTIFICATION OF PROCHLOROCOCCUS

30. In situ hybridization

31. Fred