1. Central Dogma (for your disease?) LB 145 February 22-23, 2011

2. Deoxyribonucleic Acid

3. Protein (short protein called peptide) Protein =Amino Acids stuck together By what kind of bond? What kind of reaction is this?

4. From Gene to Peptide Functional Peptide: PROTEIN

6. mRNA Synthesis of mRNA in the nucleus DNA NUCLEUS CYTOPLASM 1

7. mRNA Synthesis of mRNA in the nucleus DNA NUCLEUS mRNA CYTOPLASM Movement of mRNA into cytoplasm via nuclear pore 1 2

8. mRNA Synthesis of mRNA in the nucleus DNA NUCLEUS mRNA CYTOPLASM Movement of mRNA into cytoplasm via nuclear pore Ribosome Amino acids Polypeptide Synthesis of protein 1 2 3

13. Cystic Fibrosis is most often caused by mutation  F508 in the CFTR gene o The CFTR gene is 189,000 bases long There are 27 exons/ 26 introns in it. o The mRNA (all exons) is 6129 bases How many codons is that, max? o The protein is 1480 amino acids long Normally it’s a Cl- channel, the mutation?

14. What do you know of your disease’s Central Dogma? o What is the size of your gene? How many exons/introns does it have? o What is the size of your mRNA (cDNA)? How many codons does it have? o What is the size of your protein? What is the normal vs abnormal function of it?

15. Reminders Peer Review DUE –Might want to look over the criticisms soon-ish Order your primers by 5pm FRIDAY Check ATCC.org for cells and DNA.