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Primary Immunodeficiency Preeda Sangacharoenkit M.D. Division of Allergy and Immunology Department of Pediatrics Bangkok Metropolitan Administrative Medical.

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Presentation on theme: "Primary Immunodeficiency Preeda Sangacharoenkit M.D. Division of Allergy and Immunology Department of Pediatrics Bangkok Metropolitan Administrative Medical."— Presentation transcript:

1 Primary Immunodeficiency Preeda Sangacharoenkit M.D. Division of Allergy and Immunology Department of Pediatrics Bangkok Metropolitan Administrative Medical Colleague

2 Cooper et al. American Academy of Family Physicians November 2003.

3

4 Rosenzweig and Holland. J Allergy Ciln Immunol April 2004.

5 Evaluation of T-cell function Quantitative evaluation : T-cell subpopulation Qualitative evaluation : Delayed-type hypersensitivity (DTH) skin testing Fleisher and Oliveira. J Allergy Clin Immunol August 2004.

6 Evaluation of phagocytic function The nitroblue tetrazolium test (NBT) Fluorescent cytometry using the dihydrorhodamine-123 (DHR) probe Segal and Holland. Pediatric Clinics of North America December 2000.

7 Roos et al. Microbes and Infection 5 (2003)

8 Panel D shows the results of the nitroblue tetrazolium test in normal neutrophils: phagocytosis results in dark-blue staining of the cytoplasm. Panel E shows the results of the nitroblue tetrazolium test in neutrophils from a patient with chronic granulomatous disease: there is no phagocytosis and thus no dark-blue cytoplasmic staining. Lekstrom-Himes and Gallin. NEJM December 2000.

9 Jirapongsananuruk et al. J Allergy Clin Immunol February 2003.


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