1. DNA/RNA & Molecular Basis of Evolution ASTR 1420 Lecture 4 Sections 5.2, 5.4

2. Organic Building Blocks

3. Cells Typical Eukaryotic Cell All living organisms are made of cells All living organisms are made of cells Adult human body have 10-50 trillion cells! Adult human body have 10-50 trillion cells!

4. Components of Cell Carbohydrates (= carbs) : providing energy to cells and make cellular structures  sugars, starches, cellulose, etc. Carbohydrates (= carbs) : providing energy to cells and make cellular structures  sugars, starches, cellulose, etc. Lipids (aka fats) : store energy, make cell membranes Lipids (aka fats) : store energy, make cell membranes Proteins : versatile, essential molecules of cells Proteins : versatile, essential molecules of cells Nucleic acids : handling heritable information of cells – DNA and RNA Nucleic acids : handling heritable information of cells – DNA and RNA

5. DNA  Proteins All Earth life have DNA, and DNA is the backbone of heredity. All Earth life have DNA, and DNA is the backbone of heredity. o Heredity  DNA replication o function of DNA (genes)  protein synthesis What’s protein? What’s protein? o organic compound made of amino acids in a folded chain o essential part of organisms and participate in every process within cells  enzymes involved in matabolism  make cell stucture  cell signaling, immune responses, etc.

6. DNA structure http://www.youtube.com/watch?v=qy8dk5iS1f0

7. DNA replication http://www.youtube.com/watch?v=hfZ8o9D1tu s

8. Protein synthesis http://www.youtube.com/watch?v=XO-2Z933AzM

9. RNA world? In the beginning of Earth life, the very first life could not be based on DNA. DNA is way too complicated to be created by mere “lucky” chemical reaction. In the beginning of Earth life, the very first life could not be based on DNA. DNA is way too complicated to be created by mere “lucky” chemical reaction.  early life must have used a simpler molecule (e.g., RNA)  or, DNA was introduced externally?!? Although we do not expect DNA or RNA to appear in life on other worlds, molecules with similar function are well expected!

10. Amino Acids Most important monomer! Most important monomer! Basic building blocks of proteins, nucleotides, and sugars Basic building blocks of proteins, nucleotides, and sugars Typically, few hundred amino acids form a protein polymer. There are about 70 different amino acids in the nature, but only ~20 are used in living organisms. Typically, few hundred amino acids form a protein polymer. There are about 70 different amino acids in the nature, but only ~20 are used in living organisms. A strand of amino acids, part of a protein

11. Monomer/Polymer Glycogen: the carbohydrate that animals use to store energy is a polymer made of glucose molecule contains 22 atoms Glycogen: the carbohydrate that animals use to store energy is a polymer made of glucose molecule contains 22 atoms Most life forms consist of small number of simple molecule types called monomers, which can join together in a repetitive sequence to become larger and more complex molecules called polymers. glucose

12. Chirality of Amino Acids Chirality (handedness) Chirality (handedness) For a given amino acid, there are two geometrical shapes: left-handed & right-handed right-handed In non-biological environ: two types are equally found In non-biological environ: two types are equally found In living organisms: only left-handed amino acids are found! In living organisms: only left-handed amino acids are found!  All Earth life forms were originated from an ancestral life using only left-handed amino acids!  All Earth life forms were originated from an ancestral life using only left-handed amino acids!

13. Genes a distinct portion of DNA strand representing any single function (e.g., instruction for one protein) a distinct portion of DNA strand representing any single function (e.g., instruction for one protein) SpeciesNo. of genes Virus10 Mitochondria40 Microbe1,000 E coli4,400 Human27,000 Mouse29,000 Rice50,000

14. Small difference in genes Different human races Different human races o Any possible distinct genetic differences are now disappeared by inter-racial breed. o Genetic difference b/w races < b/w two individuals from one race! Astrobiological Implication? Astrobiological Implication? o Small genetic difference  huge difference (e.g., Chimpanzee vs. Human) ; Chimpanzee and human share 98% of the same genes o Is advanced intelligence an inevitable evolutionary outcome?

15. Genetic code : a set of rules for reading DNA Codes for most amino acids really depend on just two letters  in the past, genetic codes were two letter based… Genetic “words” consist of three DNA bases in a row. For the purpose of protein building, each word represents either a particular amino acid or “start reading” or “stop reading”. Genetic “words” consist of three DNA bases in a row. For the purpose of protein building, each word represents either a particular amino acid or “start reading” or “stop reading”. Genetic code is same for nearly ALL living organisms on Earth!  common ancestor of all life on Earth! Genetic code is same for nearly ALL living organisms on Earth!  common ancestor of all life on Earth!

16. Mutation Replicating the whole ~3 billion bases for human DNA takes only a few hours Replicating the whole ~3 billion bases for human DNA takes only a few hours About 1 replication error in billion chances About 1 replication error in billion chances o wrong base o deleted base o extra base o etc. Most of these errors have no effect Most of these errors have no effect Why? about 95% of human DNA bases are “non- coding DNA” Most significant change is from “deleted base”. Due to this “error”, every individual living organism differ each other. Due to this “error”, every individual living organism differ each other. Mutation = molecular engine of the evolutionary adaption! Mutation = molecular engine of the evolutionary adaption! Any change in the base sequence of DNA Any change in the base sequence of DNA

17. Sickle-cell disease Just one base change in one gene (Adenine  Thymine) Just one base change in one gene (Adenine  Thymine) Most mutations are harmful, but occasional good mutations are being picked up by “natural selection” Most mutations are harmful, but occasional good mutations are being picked up by “natural selection”

18. Another evidence of the common ancestor ATP (Adenosine TriPhosphate). Cells recyle ATP. The ATP molecule gives up energy when it splits into ADP and a phosphate group. ATP/ADP are like battery o ATP : fully charged o ADP : fully discharged There are many other possibilities of different molecular pairs (i.e., different batteries), but all Earth life use ATP as the energy reservoir.  common ancestor of all Earth life.

19. In summary… Important Concepts Common ancestor of all Earth Life (chirality of amino acid, common genetic code, and use of ATP among all living organisms on Earth) Some non-DNA based life forms in the beginning? DNA replication Important Terms amino acids / proteins chirality genes, genetic code mutation Chapter/sections covered in this lecture : 5.2 & 5.4