1. Characterization of Anthocyanins in Purple Pods of Phaseolus vulgaris cv. Blauhilde Brandon Lew March 24, 2007

2. Lab Work - Hamline University Goal: To identify pigments that cause the purple coloration of pod coats in Phaseolus vulgaris cv. Blauhilde Hypothesis: The purple color is caused by anthocyanins

3. Phaseolus vulgaris Kidney, black, navy, and snap beans Anthocyanins found in: –Seed coat of kidney beans Choung et al. 2003 –Seed coat of black beans Takeoka et al. 2005 Neither pod coats, not cultivar Blauhilde studied

4. Cultivar Blauhilde Pod coats purple Purple disappears when cooked

5. Anthocyanins Pigments Anti-carcinogenic Katsube et al. 2003 Anti-oxidative Tamura and Yamagami, 1994 Colorful, Good for health

6. Methods

7. High-Performance Liquid Chromatography (HPLC) Small substances Large substances

8. HPLC of Extract

9. Isoabsorbance of Extract

10. HPLC after Purification

11. Isoabsorbance after Purification

12. Mass Spectroscopy Detector FragmentsMolecule Magnets e-e-

13. Mass Spectrum of Extract

14. Identified Molecule Malvidin 3,5-diglucoside

15. Conclusion Identified anthocyanin as malvidin 3,5- diglucoside 86% of total anthocyanin content

16. Future Work Genetically engineer different anthocyanins –Keeps health benefits –Different colors

17. Acknowledgements Dr. John Matachek Hamline University Chemistry Dept. Ms. Fruen Ms. Richter Team Research

18. Characterization of Anthocyanins in Purple Pods of Phaseolus vulgaris cv. Blauhilde Brandon Lew March 24, 2007