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BODY FLUIDS DEPARTMENT OF PATHOLOGY AND LABORATORY DIAGNOSIS

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Presentation on theme: "BODY FLUIDS DEPARTMENT OF PATHOLOGY AND LABORATORY DIAGNOSIS"— Presentation transcript:

1 BODY FLUIDS DEPARTMENT OF PATHOLOGY AND LABORATORY DIAGNOSIS
FATIMA COLLEGE OF MEDICINE

2 CEREBROSPINAL FLUID

3 SPECIMEN OBTAINED BY LUMBAR PUNCTURE
CEREBROSPINAL FLUID SPECIMEN OBTAINED BY LUMBAR PUNCTURE

4 TUBE 1 = CHEMISTRY, SEROLOGY
CEREBROSPINAL FLUID TUBE 1 = CHEMISTRY, SEROLOGY TUBE 2 = MICROBIOLOGY TUBE 3 = HEMATOLOGY

5 CEREBROSPINAL FLUID TUBES OF CSF: APPEARANCE LEFT TO RIGHT IS NORMAL, XANTHOCHROMIC, HEMOLYZED AND CLOUDY.

6 NORMAL LYMPHOCYTES AND MONOCYTES, X500
CEREBROSPINAL FLUID NORMAL LYMPHOCYTES AND MONOCYTES, X500

7 CEREBROSPINAL FLUID MACROPHAGES SHOWING ERYTHROPHAGOCYTOSIS AND HEMOSIDERIN GRANULES, X500.

8 CEREBROSPINAL FLUID MALIGNANT MELANOMA CELL CONTAINING DUST-LIKE GRANULES. GRANULES ARE MUCH FINER THAN HEMOSIDERIN GRANULES. X500.

9 CEREBROSPINAL FLUID CHOROID PLEXUS CELLS: CAN BE DISTINGUISHED FROM MALIGNANT CELLS BY THE NUCLEAR UNIFORMITY AND DISTINCT CELL BORDERS. X500.

10 EOSINOPHILS: NOTICE CYTOCENTRIFUGE DISTORTION. X1000.
CEREBROSPINAL FLUID EOSINOPHILS: NOTICE CYTOCENTRIFUGE DISTORTION. X1000.

11 NUCLEATED RED BLOOD CELLS SEEN WITH BONE MARROW CONTAMINATION. X1000.
CEREBROSPINAL FLUID NUCLEATED RED BLOOD CELLS SEEN WITH BONE MARROW CONTAMINATION. X1000.

12 BROAD SPECTRUM OF LYMPHOCYTES SEEN IN VIRAL MENINGITIS. X1000.
CEREBROSPINAL FLUID BROAD SPECTRUM OF LYMPHOCYTES SEEN IN VIRAL MENINGITIS. X1000.

13 CEREBROSPINAL FLUID NEUTROPHILS WITH INTRACELLULAR BACTERIA SEEN IN BACTERIAL MENINGITIS. X1000.

14 CEREBROSPINAL FLUID LYMPHOMA CELLS. NOTICE PROMINENT NUCLEOLI AND FINE NUCLEAR CHROMATIN. X1000.

15 CEREBROSPINAL FLUID MYELOBLASTS FROM ACUTE MYELOCYTIC LEUKEMIA. NOTICE PROMINENT NUCLEOLI. X1000.

16 CEREBROSPINAL FLUID MEDULLOBLASTOMA: NOTICE CLUSTERING OF CELLS, NUCLEAR MOLDING, AND ROSETTE FORMATION. CLUSTERING AIDS IN THE DIFFERENTIATION OF THESE CELLS FROM LYMPHOBLASTS. X1000.

17 GRAM STAIN OF CRYPTOCOCCUS NEOFORMANS. X1000.
CEREBROSPINAL FLUID GRAM STAIN OF CRYPTOCOCCUS NEOFORMANS. X1000.

18 CEREBROSPINAL FLUID INDIA INK PREPARATION OF CRYPTOCOCCUS NEOFORMANS: NOTICE BUDDING YEAST FORM. X400.

19 SYNOVIAL FLUID POLARIZED URIC ACID CRYSTALS; INTRACELLULAR NEEDLE-SHAPED CRYSTALS. X500.

20 SYNOVIAL FLUID WRIGHT’S STAINED CELLS CONTAINING CALCIUM PYROPHOSPHATE CRYSTALS. X1000.

21 SYNOVIAL FLUID POLARIZED CALCIUM PYROPHOSPHATE CRYSTALS; INTRACELLULAR RHOMBIC-SHAPED CRYSTALS. X1000.

22 NORMAL MESOTHELIAL CELLS, LYMPHOCYTES, AND MONOCYTES. X500.
PLEURAL FLUID NORMAL MESOTHELIAL CELLS, LYMPHOCYTES, AND MONOCYTES. X500.

23 PLEURAL FLUID ONE NORMAL AND TWO REACTIVE MESOTHELIAL CELLS WITH MULTINUCLEATE FORM. X1000.

24 PLEURAL FLUID LUPUS ERYTRHEMATOSUS (LE) CELL; NOTICE THE INGESTED “ROUND BODY”. X1000.

25 PLEURAL FLUID METASTATIC CELLS FROM BREAST CANCER. A CLASSIC “CANNONBALL” FORMATION IS SEEN UNDER LOW POWER AIDING IN DIFFERENTIATING THESE CELLS FROM MESOTHELIAL CELLS. X1000.

26 PLEURAL FLUID ADENOCARCINOMA SHOWING FINE NUCLEAR CHROMATIN, NUCLEAR AND CYTOPLASMIC MOLDING, AND VACUOLATED CYTOPLASM. X1000.

27 SMALL CELL CARCINOMA SHOWING NUCLEAR MOLDING. X250.
PLEURAL FLUID SMALL CELL CARCINOMA SHOWING NUCLEAR MOLDING. X250.

28 COLON CARCINOMA SHOWING MUCIN-CONTAINING VACUOLES. X500.
PERITONEAL FLUID COLON CARCINOMA SHOWING MUCIN-CONTAINING VACUOLES. X500.

29 THANK YOU!!!


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