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Surface plasmon resonance imaging detection of silver nanoparticle-tagged immunoglobulin by Sharmistha Paul, Deepen Paul, George R. Fern, and Asim K. Ray.

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Presentation on theme: "Surface plasmon resonance imaging detection of silver nanoparticle-tagged immunoglobulin by Sharmistha Paul, Deepen Paul, George R. Fern, and Asim K. Ray."— Presentation transcript:

1 Surface plasmon resonance imaging detection of silver nanoparticle-tagged immunoglobulin by Sharmistha Paul, Deepen Paul, George R. Fern, and Asim K. Ray Interface Volume 8(61):1204-1211 August 7, 2011 ©2011 by The Royal Society

2 Schematic of surface plasmon resonance imaging (SPRI). Sharmistha Paul et al. J. R. Soc. Interface 2011;8:1204-1211 ©2011 by The Royal Society

3 Transmission electron microscopy (TEM) images of (a,b) AgNPs and (c) AgNP-functionalized anti-gIgG (AgNP–anti-gIgG) using a 200-mesh TEM grid as the mask. Sharmistha Paul et al. J. R. Soc. Interface 2011;8:1204-1211 ©2011 by The Royal Society

4 Size distribution histograms of (a) AgNPs and (b) AgNP–anti-gIgG from the dynamic light scattering experiments. Sharmistha Paul et al. J. R. Soc. Interface 2011;8:1204-1211 ©2011 by The Royal Society

5 UV–visible absorption spectra of 12.46 µg ml−1 AgNPs (dotted line), 11.9 mM anti-gIgG (dashed- dotted line) and AgNP–anti-gIgG (solid line) solution (diluted three times). Sharmistha Paul et al. J. R. Soc. Interface 2011;8:1204-1211 ©2011 by The Royal Society

6 Comparison of SPRI reflectivity spectra of bare Au substrate (filled squares), modification with MUA (filled circles) and anti-goat immunoglobulin G (gIgG) (filled triangles) immobilization. Sharmistha Paul et al. J. R. Soc. Interface 2011;8:1204-1211 ©2011 by The Royal Society

7 SPR image of the microarray goat immunoglobulin G (gIgG) spot: (a) 6.66 nM, (b) 660 nM at 552 nm (intensity in greyscale is shown at the bottom of the pictures) and (c) spatial distribution of reflectivity (%) of the gIgG spot in the microarray (solid and b... Sharmistha Paul et al. J. R. Soc. Interface 2011;8:1204-1211 ©2011 by The Royal Society

8 Response of Δ%R of immobilized anti-goat immunoglobulin G (gIgG) against the concentration of goat immunoglobulin G (gIgG) within the range of 6.66–660 nM. Sharmistha Paul et al. J. R. Soc. Interface 2011;8:1204-1211 ©2011 by The Royal Society

9 SPR images of the microarrayed AgNP–anti-goat immunoglobulin G (gIgG) spot of (a) 6.66 nM and (b) 660 nM at 552 nm and (c) the spatial distribution of the reflectivity (%) of the spots (solid and broken lines for 6.66 and 660 nM, respectively). Sharmistha Paul et al. J. R. Soc. Interface 2011;8:1204-1211 ©2011 by The Royal Society

10 Change in percentage of reflectivity (Δ%R) for different concentrations of goat immunoglobulin G (gIgG) with and without AgNPs at an SPRI wavelength of 552 nm (average over three measurements). Sharmistha Paul et al. J. R. Soc. Interface 2011;8:1204-1211 ©2011 by The Royal Society


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