1. Trichomoniasis Field Perspectives Jeremy VanBoening, DVM

4. Trichomoniasis Involvement Clinical Practice Nebraska Cattlemen-NCBA Nebraska Veterinary Medical Association

5. Sample Collection Texas A&M Trich Sample Collection Video

6. Sample Collection Challenges Survey of 10 D-Labs 10-14-2013

7. Summary of PCR Related Questions IncubationYes= 2No=6Your Choice=2 Ship on IceYes=2Room Temp=6Other=2 PoolingUp to 5=7No Pooling=2Up to 3=1 Preferred Collection MediaIn Pouch=6Saline=2 Tubes=2

8. Sample Collection Challenges Restraint and Safety

9. Is this safe??

11. What Collection Method 1.preputial scraping with pipette 2.preputial scraping using thrichit 3.saline rinse 4.swap or scraping of the penis NO BLOOD for Either PCR or Culture

12. Sample Collection Challenges Cleanliness Field Collection Area Scissors (Disinfect or Not and with what?) Access to saline for rinsing

13. Dirty Samples Rarely if Ever Return Positive Recent Study Hustun, etal. MSU – 20 Samples w/ bacteria injected at increasing #’s – 20 Samples clean (Only T. Foetus Added) – 0 of 20 with bacteria ever recorded as positive – 20 of 20 w/out bacteria had at least one positive recording

14. Sample Collection Challenges Proper Collection Media

15. Sample Preparation  Depends on the D-Lab!!  Collection Medium (In-Pouch, Tubes, Saline)  Incubate or Not Depends on D-Lab, PCR or Culture,  How should the sample be packaged? On Ice, Room Temperature, Or Depends on the Weather

16. Transportation Issues Rural Access to Timely Reliable Transportation How to package and send samples? – How long can they be in transit and at what temperature? – Sensitivity and Specificity go down for both culture and PCR Hustun etal MSU

17. How Do We Get Better???? Standardization 1.Adopt the Best Diagnostic Testing Available (qPCR) Using Chemical-Lysis and IAC. 2.Promote SOP’s for Sampling, Sample Prep, and Shipping 3.Increased Veterinarian/Producer Awareness 4.Standardized Regulations