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Published byReynold Fitzgerald Modified over 9 years ago
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Extraction of Nicotine By: DJ Hall and Brittany Hicks
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Purpose Determine if nicotine is extractable by means of supercritical fluid Run more than one sample type to determine if this makes a significant difference
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SFE Supercritical Fluid Extraction Uses high pressure CO 2 Used to extract organics from different types of matrixes Allows for easy separation Problems All soluble organics will be separated form matrix
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GC-MS Gas Chromatography Mass Spectroscopy Separation of violate organics Determine the analyte separated at specific retention times using MS
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Nicotine (162.12g/mol) Poison (parasympathomimetic alkaloid) Formed in nightshade family of plants Antiherbivor chemical Small doses Stimulant Relaxation Large doses Fatal
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Extraction 2 Cigarettes Marlboro blend 27 2 Pieces Nicorette Gum 2mg 50 Drops of e-cigarette liquid 2.4% by weight
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Extraction cont. All extractions were obtained by bubbling into methanol and using SFE methanol modifier pump Cigarettes Ground using mortar and pestle Nicorette Gum Cut into small pieces E-cigarette liquid Celite as inert matrix
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Determination of Nicotine All samples were diluted to 50ml of methanol A standard was created by diluting 4mg of Nicotine in 50ml of methanol Standards and Samples were run using the GC-MS
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Reference Mass Spectra
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Standard Nicotine peak
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Cigarettes Nicotine peak
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Cigarettes cont.
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E-Cigarette Liquid Nicotine peak
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E-Cigarette Liquid Cont.
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Nicorette Gum
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Chromatogram Comparison E-Cigarette Liquid Nicorette Gum Cigarette Standard
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Conclusion It is possible to extract nicotine using the SFE Both the E-Cigarette and Cigarette samples contained nicotine The Nicorette Gum showed undetectable amounts of nicotine Nicotine may have be contained in the gum residue that was filtered before dilution
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Future Project Quantify the amounts of nicotine extracted Determine the extraction efficiency
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Work Cited Fischer, M. and Jefferies, T. Optimization of Nicotine Extraction from Tobacco Using Supercritical Fluid Technology with Dynamic Extraction Modeling. J.Agric. Food Chem. (1996), 44, 1258-1264.
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