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Dr. ZherdevaV., Dr. Loginova Y., Dr. Kazachkina N., Dr. Savitsky A. Bach Biochemistry Institute of Russian Academy of Science Moscow 2013
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Semiconducter core: Cd/Se, Cd/Te, и Ga/N shell: Zn/S, Cd/Se biofunctional coating: polymer, protein or lipid Excitation in broad range of spectrum emission depends on size Lifetime of 20-40 ns high extinction coefficient high quantum yeild uniqe photostability - Semicondacter nanocrystal (1-10 nm) with unique photophysical properties :
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APPLICATION OF QDS Michalet X, Pinaud FF, Bentolila LA, Tsay JM, Doose S, Li JJ, Sundaresan G, Wu AM, Gambhir SS, Weiss S: Quantum Dots for Live Cells, in Vivo Imaging, and Diagnositics. Science 2005 307(5709): 538-544.
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QDs is a perspective tools for medical usage… TOXIC or NON TOXIC? Distribution Localization and accumulation in vivo. Distribution Localization and accumulation in vivo. per osIntravenously (i.v.)
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TECHNOLOGY Type of administration I.V. Per os I.V. Per os Localization study In vivo imaging Local fluorescence spectroscopy Confocal fluorescent microscopy Pathomorphogy Morphology Clinical biochemistry Toxical effects
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QDs MPA λ em 611nm and 630 nm d ~ 8 – 11нм QY -10-20% QDs PolyT λ em 626 нм d ~ 15 – 16 нм QY 10-30% QDs PolyT-APS λ em 678 нм d ~ 36 нм QY 5-20% QY
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FLUORESCENT EQUPMENT iBox UVP Laser spectrometer with optical zond
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Monitoring of fluorescence in vivoin digestive tract of mice Excitation 502-547 nm, emission filter 570-640 nm exposition25с. per os
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QDs Stomach after 2 h Intenstines after 2 h Intenstines after 4 h Intenstines after 6 h MPA+--- PolyT++±- PolyT-APS++++ No fluorescence (-), weak (±), high (+). Local fluorescense spectroscopy Fluorescence of excrements after 24 h of QDs adminestering per os QDs MPA Black line- excrements of control mice Red line- excrements of after QDs administering QDs PolyT-APS QDs PolyT
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I.V. QDs MPA LFS
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QDs Poly T LFS I.V.
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i.V. QDs PolyT-APS LFS
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Organs Heart Lungs Liver Spleen Kidney Brain Limp nodes М uscels Skin Intestines Fluorescence after 40 min +*+++ ++ +* + Fluorescence after 22 days -+++++ +-+--+ Confocal microscopy NB : weak +, medium ++, high +++, no -. (*) in blood vessel only а – reflecte lights, б – fluorescence, в – peudo color imaging, г – fluorescence spectra Lungs Liver а Lungs
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а – local haemorragia; б – stenosis of arteria. Blood Urea creatinine, Morphology Toxicity of QDs MPA No change alkaline phosphatase alanine - and aspartate transaminases Hihg spleen weight (twice ) Pathomorphology а б
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I.V. QDs MPA toxicity: also depends on initial solution
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1.Distribution and accumalation depend on the modification of coating 2.Qds are dissolving in digestive tract: PolyT-APS are more stable comparing to QDs МPА and PolyT after per os administering 3.The main targeted organs for QDsМPА are the lungs, for PolyТ are the atria, for PolyT-APS are liver after I.V. Excretion of in the urine and faeces was not detected. 4.QDs of small size МPA stay in organism for a long period.They could lead to long-term pathomorphological changes in lungs on the 22-th day after administration. 5. The the severity of the toxic effect of QDs MPA depends on the solvent CONCLUSION
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Локальная флуоресцентная спектроскопия (ЛФС)
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