1. RESEARCH AND TECHNOLOGY TRANSFER AT THE BIO/NANO/ELECTRONIC INTERFACE Applied Biotechnology Innovation Centre Ioanis Katakis Octoner 25, 2004 Tarragona

2. Applied Biotechnology Innovation Centre WHO ARE WE? 1994 19951996199719981999 2000 20012002 200320042005 Students PhD Technicians BBCAT Evolution of membership

3. Applied Biotechnology Innovation Centre HOW WE FINANCE OUR WORK? 1994 19951996199719981999 2000 20012002 200320042005 private public BBCAT

4. Applied Biotechnology Innovation Centre HOW WE WORK? Knowledge Creation Biosensors Bioelectronics Nanotechnology Microsystems (Photo)catalysis Biotechnology Technology Transfer Clinical & veterinary diagnostics Biomedical applications Food & Agriculture Pharmaceutical & Fine Chemicals Chemical Industry Renewable energies Innovation Spin off companies New educational/vocational models

5. Applied Biotechnology Innovation Centre OUR SECOND RESEARCH PLAN MICROSYSTEMS Enzyme (NAD) BIOELECTRONICS NANOTECHNOLOGY BIOSENSORS NEW AREAS APPLIED RESEARCH Immunosensors DNA Biofuel cellsNeuron chip Directed depositionNanocatalysts Screen printedMicroreactors Aptamers Bioreactors Solar Cells Making immuno strips and systems

6. Applied Biotechnology Innovation Centre FROM SUB MOLECULAR TO MACRO GENES PROTEINS CELLS TISSUES ORGANS ORGANISMSECOSYSTEMSNANOTECHNOLOGY BIOSENSORS BIOELECTRONICS TISSUE ENGINEERING IN VITRO SYSTEMS MICROSYSTEMS MULTI MICRO BIOREACTORS ENVIRONMENTAL BIOTECHNOLOGY MATERIALS AND CHEMISTRY

7. Applied Biotechnology Innovation Centre SOME RESEARCH LINES (1/4) SELF ASSEMBLY FOR THE ULTIMATE (BIO)CHEMICAL CATALYTIC FLEXIBILITY Narváez et al. Biosens Bioelectr 15:43-52 (2000) Narváez et al. J Electroanal Chem 430:227-33 (1997)

8. Applied Biotechnology Innovation Centre SOME RESEARCH LINES (2/4) SUPRAMOLECULAR ARCHITECTURES FOR COMPLEX BIOSENSING, AMPLIFICATION, AND CATALYSIS TASKS Popescu et al. J Electroanal Chem 464:208-14 (1999) (+2 in preparation) V. Pavlov Thesis (2005) on biosensors, K. Horton DEA (2003) liposomes Sonicate, extrude

9. Applied Biotechnology Innovation Centre SOME RESEARCH LINES (3/4) Campàs & Katakis Int J Env Anal (in press), PCT&US pat. Appl (2002) M. Campàs PhD Thesis (2003), S. Dondapati DEA Thesis (2004) gold colloid oligonucleotide colloidal gold- oligonucleotide selective deposition deposition conjugation NANOCOLLOID SYNTHESIS AND MODIFICATION FOR NANOPATTERNING AND ARTIFICIAL INTELLIGENCE

10. Applied Biotechnology Innovation Centre SOME RESEARCH LINES (4/4) Os+ - - - - - - + + + + + + - - - - - - }N1}N1 + + + + + + + + + + + + }N2}N2 - - - - - - Glucose oxidase produces H 2 O 2 Peroxidase uses H 2 O 2 and consumes electrons at E 2 Glucose oxidase produces electrons at E 1 e-e- e-e- P. Pescador DEA thesis (2004) Biofuel cells TOWARDS THE ULTIMATE NANOMACHINE(?): SELF POWERED, SELF PROPELLED, SELF PROPAGATING

11. Applied Biotechnology Innovation Centre BBG/DINAMIC MOST VALUABLE RESOURCE Magda Constanti (Lector, Microbiology) Teresa Hechevarria (Torres Quevedo, Chemical Engineering) Ioanis Katakis (Associate professor, Chemical Engineering) Petros Lenas (Ramon y Cajal, Tissue Engineering) Przemyslaw Los (Visiting Professor, Physical Chemistry) Josep Ma Montornés (Post doctoral fellow, Org. Synthesis) Susana Rodríguez (Ramon y Cajal, Bioprocess Engineering) Jose Luis Toca-Herrera (Ramon y Cajal, Nanotechnology) SENIOR MEMBERS AND PROJECT MANAGERS

12. Applied Biotechnology Innovation Centre BBG/DINAMIC MOST VALUABLE RESOURCE PERMANENT TECHNICIANS AND SUPPORT Barbara Vastenavond (Administration) Lea Gragert (Administration and accounting) Mònica Mir (Technician, Electrochemistry) Joaquim Solana (Marketing, Project support) Diego Bejarano (Technician, Chemical & Process Engineering) Marta Jimenez (Technician, microbiology) MireiaMassanell (Technician, Immunology) Cristina Sanz (Technician, Analytical techniques) Xavier Roch (IT Technician)

13. Applied Biotechnology Innovation Centre BBG/DINAMIC MOST VALUABLE RESOURCE GRADUATE STUDENTS AND TRAINEES Mònica Mir (PhD Student, Bioelectrochemistry) Srujan Dondapati (PhD Student, Bioelectrochemistry) Paula Pescador (PhD Student, Molecular biology/fuel cells) Veronica Saravia (PhD Student, Tissue engineering) Viviana Duarte (PhD Student, Immunosensors) Frank Jeyson (PhD Student, Biofuel cells) Kelly Briceño (PhD Student, Bioelectrochemistry) Peter Pfeiffer (PhD Student, Biomolecular interactions) Rory McNally (MS trainee, Microbiology) Denise Hogan (MS trainee, Microbiology) Antoni Montseny (Undergraduate trainee) David Romero (Undergraduate trainee) Josep Pamies (Undergraduate trainee)

14. Applied Biotechnology Innovation Centre OUR PRODUCTIVITY IN PERSPECTIVE PhD Theses:2,0 (1995-2004) DEA Theses:9,0 (1995-2004) Publications/PDI/year:2,5 (ISI 1990-1999) Citations/PDI/year:21,0(ISI 1990-1999) DATA BBG DATA Catalunya (all areas) Citations/article/year:1,7 (1994-1998) DATA USA (Chemical Engineering source: NRC 1990-94) Citations/PDI/year:23,44 (rank #1 U of Minnesota) 20,78 (rank #2 Stanford U) Publications/PDI/year:4,6(rank #1 U of Minnesota) 2,5(rank #13 SUNY, Buffalo) 1,7(rank #72 U of Tulsa)

15. Applied Biotechnology Innovation Centre ACKNOWLEDGEMENTS Our work is financed by: INNOCUOUS (Immunostrips for TCA detection) MICROPROTEIN (Patterning and arraying) HEALTHY AIMS (Fuel cells) CIDEM and our Clients URV, DURSI, MECD