1. Nomenclature

2. Reactions  Substrates (reactants) -- A B C D  Products -- P Q R S  Enzyme -- E F G  Inhibitors -- I J  Activators  Metal ion -- M n+/-  Other -- X Y Z  Substrates (reactants) -- A B C D  Products -- P Q R S  Enzyme -- E F G  Inhibitors -- I J  Activators  Metal ion -- M n+/-  Other -- X Y Z

3. Enzymes  Stable: when isolated from the rest of the reaction components, it has a long half-life with respect to the assay times scale, and reactants add to it in a bimolecular step.  Transitory: when isolated from the rest of the reaction components its half-life is short relative to the assay time scale.  Central: if the active site is completely filled and kinetically competent  Noncentral: if not.  Stable: when isolated from the rest of the reaction components, it has a long half-life with respect to the assay times scale, and reactants add to it in a bimolecular step.  Transitory: when isolated from the rest of the reaction components its half-life is short relative to the assay time scale.  Central: if the active site is completely filled and kinetically competent  Noncentral: if not.

4. Kinetic Mechanism  Sequential: all reactants must be bound to an enzyme before any reaction occurs.  Ping-Pong: a product is released between the addition of two substrates  Ordered: there is an obligatory order fo addition of reactants to an enzyme and /or release of products from enzyme.  Random: there is not.  Sequential: all reactants must be bound to an enzyme before any reaction occurs.  Ping-Pong: a product is released between the addition of two substrates  Ordered: there is an obligatory order fo addition of reactants to an enzyme and /or release of products from enzyme.  Random: there is not.

5. Kinetic Constants  Michaelis constant (Km) is defined as the concentration of reactant that gives half the maximum rate at saturating concentrations of all other reactants and with products maintained at zero concentration.

6. Rate Constants  Microscopic: associated rate constants of the individual steps in a reaction scheme  First-order  Second-order  Macroscopic:  Microscopic: associated rate constants of the individual steps in a reaction scheme  First-order  Second-order  Macroscopic:

12. Michaelis-Menten