1. GENETICAL FEATURES OF CHROMOSOMAL FORM «C» OF MICROTUS MAXIMOWICZII FROM THE RUSSIAN FAR EAST GENETICAL FEATURES OF CHROMOSOMAL FORM «C» OF MICROTUS MAXIMOWICZII FROM THE RUSSIAN FAR EAST Irina V. Kartavtseva, Irina N. Sheremetyeva Institute of Biology & Soil Science, Far Eastern Branch of Russian Academy of Sciences, Vladivostok (Russia) Anastasia B. Ryabkova Khabarovsk Antiplague Station, (Russia) Svetlana A. Romanenko, Olga L. Gladkikh Institute of Molecular and Cellular Biology SB RAS (Russia) Lubov V. Frisman Institute of Complex Analysis of Regional Problems Far Eastern Branch of Russian Academy of Sciences, Birobidzhan (Russia)

2. The role of chromosomal rearrangements in speciation still unclearly. The maximowicz's vole Microtus maximowiczii (Rodentia) is convenient species for investigating this problem. Introduction

3. Rearrangements by Mejer et al., 1996 1 - is fusion of two middle sized pairs of metacentrics to formating of large metacentric. All rearrangements for this species can be divided into three types: 2 - is Robertsonian rearrangements. 3 - is pericentric inversions of 4 pairs.

4. Areal and terra typica points of M. maximowiczii M. m. maximowiczii Schrenck 1858, Amurskii and Khabarovskii regions in Russia, and in northeastern China M. m. ungurensis Kastchenko 1913, Buryatia and Chitinskii regions in Russia and North-Eastern Mongolia China Mongolia Russia Korea

5. For this species was described five chromosomal forms (Kartavtseva et al., 2008): A : 2n = 39 - 44, NF = 53 - 60 (Buryatiya), B: 2n = 38 - 42, NF = 54 - 57 (Buryatiya), C: 2n = 39 - 41, NF = 55 - 60 (Far East) D: 2n= 40=43, NF= 54-58 (Zabaikalskii kray and E. Mongolia) V: 2n = 36 - 40, NF = 52 - 56 (Transbaikalia and W.Mongolia).

6. Russia Mongolia China «B» 2n=38-42 «V» 2n=40-43 «A» 2n=39-44 «D» 2n=40-43 «C» 2n=39-41 5 chromosomal forms of M. maximowiczii (Kartavtseva et al., 2008) M. m. maximowiczii M. m. ungurensis

7. M. m. maximowiczii "A“ 2n=39-44 "C“ 2n=39-41

8. control region mtDNA А С D B V “Восточная” “Южная” “Западная” M. m. ungurensis M. m. maximowiczii

9. Material Map of chromosomal investigation We investigated karyotype of 36 individuals of Maximowicz's vole from six earlier not investigated populations of the territory Middle Amur river (chromosomal form C). Literature data: 2n=42 2n=39 Our data: (2n=40-41) New data

10. 2n=40-41 2n=40 2n=41

11. Chromosomal forms “D” and “C” 2n=402n=42

12. New chromosomal nomenclature of M. maximowiczii by fluorescent in situ hybridization (FISH) M. maximowiczii FISH by Lemskaja et al., 2010 from chromosomal form “A” M. maximowiczii (#2350) from Romanovka (chromosomal form “A”, our data) 11 11,20 20 2n=41

13. M. Maximowiczii (#2060) from Norskii reservation (our data) 20 11

15. Map of investigation of control region A B mt DNA 940 np A A B

16. Conclusion In the territory Middle Amur river (chromosomal form C): Stabilization of karyotype with Robertsonian rearrangement chromosomal pairs 11 and 20 and inversion of pair 14 Two clasters of control region of mt DNA can be results of isolations of two animals groups.

17. Thanks for attention