1. John C. Walker, Director, Interdisciplinary Plant Group Bioenergy Summit, April 17, 2009 How Plant Biology Research at MU Is Helping the U.S. Achieve Its Bioenergy Goals From Plants to the Pump ipg.missouri.edu

2. Biofuels EthanolBiodiesel

3. Missouri: Ethanol fr. Corn National rank in corn production: 9th Operating ethanol plants: 6 Annual ethanol production: 275 million Percentage of all gasoline sales blended with ethanol: 70% --Source: www.mocorn.org

4. Missouri: Soy Biodiesel National rank in soybean production: 7th “Home” of soy biodiesel Operating soy biodiesel plants: 4 Annual soy biodiesel production: 90 million --Sources: National Biodiesel Board, MSMC, NASS

5. Interdisciplinary Plant Group Biochemistry Bioinformatics Cell & Molecular Biology Development Ecology & Evolution Environmental Interactions Genetics & Plant Breeding Genomics and Proteomics Plant Pathology & Physiology

6. Thelen Lab Biochemistry Genomics and Proteomics Plant Physiology

7. seed filling embryogenesismaturation development soybean canola castor starch protein oil Using advanced proteomics to show how plant cells regulate oil:protein balance.

8. Sharp Lab Environmental Interactions Genomics and Proteomics Plant Physiology

9. Genetic diversity in soybean under drought stress

10. Genetic diversity and increased root proliferation are associated with greater water uptake from drying soil. cv 1cv 2

11. Nguyen Lab Environmental Interactions Genomics and Proteomics Genetics & Plant Breeding

12. Genetic mapping and engineering for total oil and oleic acid content Metabolite profiling to understand oil composition and accumulation in soybeans Genetic factors regulating fatty acid synthesis and total oil content Oil Improvement High seed oil content + high oleic acid = better “biodiesel”

13. Genetics Plant Breeding Sleper and Shannon Labs

14. Number of lines Oil % 9.3 10.0 10.7 11.4 12.1 12.8 13.5 14.2 14.9 15.6 16.3 17.0 17.7 18.2 Parent 2Parent 1 Oil content distribution in 250 F 7 -RILs

15. Oil Quality

16. Environmental Interactions Plant Physiology New Crops for Bioenergy Fritschi Lab

17. Sweet Sorghum as a Potential Biofuel Crop

18. Environmental Interactions Plant Physiology New Crops for Bioenergy Blevins Lab

19. NAD-ME Pearl millet Amaranth Bermuda grass Switch grass Buffalo grass Plant Metabolic Pathways NADP-ME Big bluestem Indian grass Little bluestem Crab grass Corn Sorghum Sugar cane PEP-CK Guinea grass Rhode grass Side oats grama

20. Hydroponically Grown Pearl Millet and Purple Amaranth (μM Mn ) 100502510075100502510075

21. Biotechnology for Bioenergy Birchler Lab

22. Smallest mini-B chromosome Red: Transgene Green: B repeat “Minichromosomes” Targeting B chromosomes

23. Minichromosomes can serve as a platform for multiple transgenes. An extra chromosome would allow plants to be used as a factory for the production of multiple foreign proteins. Minichromosomes could also be used to add/modify whole biochemical pathways to plants.

24. Plant Transformation Facility Biotechnology for Bioenergy Zhang Lab

25. Pipeline for Biofuel Research at Plant Transformation Facility Developing efficient transformatio n systems for biofuel crops Rationale designs for regulation of lignin biosynthetic pathway Transforming biofuel crops with transgenes for enhancing biofuel production Molecular and phenotypic analysis of transgenic biofuel crops Phase 1 Phase 2 Phase 3 Phase 4 Corn/Switchgrass/Poplar Analysis of biofuel production efficiency Phase 5

26. Biotechnology for Bioenergy Oliver Lab, USDA-ARS

27. Switchgrass and Transgenes: Need for Total Gene containment Desired trait + Blocked Floral Inhibitor Activator Line Vegetative Growth Only Increased biomass + gene containment (Credit: Stephen Ausmus) Photo: Anna Gardner Active Floral Inhibitor

28. Plant Sciences @ MU