2. Live food aquaculture training course www.aquatrain.org Demonstration of Artemia decapsulation

3. Live food aquaculture training course www.aquatrain.org Background Alveolar layer of the cyst are removed

4. Live food aquaculture training course www.aquatrain.org Why decapsulated cysts? A complete contamination free and sterile product Separation problems after hatch are avoided

5. Live food aquaculture training course www.aquatrain.org Preparation Tank set-up with stainless steel filter and aeration system Addition of water and strong aeration

6. Live food aquaculture training course www.aquatrain.org Hydration Dry cysts are hydrated in water with strong aeration

7. Live food aquaculture training course www.aquatrain.org Addition of chlorine and caustic soda => increases pH above 10 Addition of chlorine and hypoclorite solution Temperature should be maintained within 25- 30  C

8. Live food aquaculture training course www.aquatrain.org Colour change 1.Dark brown 2.Grey 3.Orange The process is stopped immediately after orange colour appear Process lasts from 5-15 min

9. Live food aquaculture training course www.aquatrain.org Checking of bouyancy Decapsulated cysts will sink Non-decapsulated cysts will float

10. Live food aquaculture training course www.aquatrain.org Termination of process Process is stopped by adding large amounts of water until the smell of chlorine has disappeared Hypoclorite residue absorbed by the cysts is neutralized by 0.1 % Sodium Thio-sulfate

11. Live food aquaculture training course www.aquatrain.org Transfer to hatching tank