1. Lecture 11 Based on Chapter 17 - Regulation of Gene Expression in Bacteria and Bacteriophages Copyright © 2010 Pearson Education Inc.

2. 1 - Introduction

3. 2 – The lac Operon of E. coli

4. 3 – Lactose as a carbon Source for E.coli

5. 4 - Experimental Evidence for the Regulation of lac Genes The experiments of Jacob and Monod produced an understanding of arrangement and control of the lac genes.

6. 5 – Mutations in the Protein-Coding Genes

7. 6 – Mutations affecting the Regulation of Gene Expression

8. 7 – Operator Mutations

9. 8 – lacI Gene Regulatory Mutations

10. 9 – Jacob & Monod’s Operon Model for the Regulation of lac Genes Figure 17.5 Figure 17.6

11. 10 – Induction of lac by Allolactose (lactose)

12. 11a – Effect of lac Oc Mutations (merodiploid)

13. 11b – Effect of lac Oc Mutations (merodiploid)

14. 12a – Effect of lacI - Mutations

15. 12b – Effect of lacI - Mutations

16. 12c – Effect of lacI - Mutations

17. 13 – Additional lacI Mutations

18. 14 – Positive control of the lac Operon

19. 15 – Molecular Details of lac Operon Regulation

20. 16 - The trp Operon of E. coli

21. 17 – Molecular Model for Attenuation

22. 18 – Molecular Model for Attenuation

23. 19 – Genetic Evidence for Attenuation

24. 20 – Attenuation at Other Operons

25. 21 – The ara Operon of E. coli: Positive and Negative Control

26. 22 – Regulation of Gene Expression in Phage Lambda

27. 23 – Early Transcription Events

28. 24 – The Lysogenic Pathway

29. 25 – The Lytic Pathway