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Lecture 11 Based on Chapter 17 - Regulation of Gene Expression in Bacteria and Bacteriophages Copyright © 2010 Pearson Education Inc.

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Presentation on theme: "Lecture 11 Based on Chapter 17 - Regulation of Gene Expression in Bacteria and Bacteriophages Copyright © 2010 Pearson Education Inc."— Presentation transcript:

1 Lecture 11 Based on Chapter 17 - Regulation of Gene Expression in Bacteria and Bacteriophages Copyright © 2010 Pearson Education Inc.

2 1 - Introduction

3 2 – The lac Operon of E. coli

4 3 – Lactose as a carbon Source for E.coli

5 4 - Experimental Evidence for the Regulation of lac Genes The experiments of Jacob and Monod produced an understanding of arrangement and control of the lac genes.

6 5 – Mutations in the Protein-Coding Genes

7 6 – Mutations affecting the Regulation of Gene Expression

8 7 – Operator Mutations

9 8 – lacI Gene Regulatory Mutations

10 9 – Jacob & Monod’s Operon Model for the Regulation of lac Genes Figure 17.5 Figure 17.6

11 10 – Induction of lac by Allolactose (lactose)

12 11a – Effect of lac Oc Mutations (merodiploid)

13 11b – Effect of lac Oc Mutations (merodiploid)

14 12a – Effect of lacI - Mutations

15 12b – Effect of lacI - Mutations

16 12c – Effect of lacI - Mutations

17 13 – Additional lacI Mutations

18 14 – Positive control of the lac Operon

19 15 – Molecular Details of lac Operon Regulation

20 16 - The trp Operon of E. coli

21 17 – Molecular Model for Attenuation

22 18 – Molecular Model for Attenuation

23 19 – Genetic Evidence for Attenuation

24 20 – Attenuation at Other Operons

25 21 – The ara Operon of E. coli: Positive and Negative Control

26 22 – Regulation of Gene Expression in Phage Lambda

27 23 – Early Transcription Events

28 24 – The Lysogenic Pathway

29 25 – The Lytic Pathway


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