1. Spectrum’s mission is to provide quality Membrane Separations Technology to the Life Science market

2. Tangential Flow Filtration Solutions Tangential Flow Filtration Shanghai, Oct. 26, 2011 Yingqing Huang, General Manager for Greater China

3. Introduction to TFF Applications Challenges Our Offerings –Membranes, mPES, –System, KRIIi –Single Use Technology Designed for Scalability Summary Overview

4. Hollow Fiber TFF Effective Reduction of Membrane Fouling Tubular Membrane Geometry Large Surface Area in a Compact Size Gentle Separations with Relatively Low Pressure Available in UF and MF pore size ranges Directly Scalable by Increasing Number of Fibers

5. Applications Alternatives to centrifugation, depth filtration, etc. Separation Purification Sterilization Buffer exchange Concentration

6. Company Application MERCK Drug delivery, vaccines ACCELERON PHARMA Theoepeutical rProteins, IgG NOVARTIS VACCINES & DIAGNOSTICS E.Coli concentration SAIC FREDERICK Vaccines BAXTER HEALTHCARE CORP. Pertusis, Mening C MEDIMMUNE Vaccines ABBOTT LABORATORIES Diagnostics INTEGRA BIOLOGICS Bone Repair AMGEN, INC. Cell Perfusion ROCHE DIAGNOSTICS Diagnostics, latex NANOSPECTRA BIOSCIENCES, INC. Gold nanoparticles BECKMAN COULTER, INC. Diagnostics GENENTECH INC Cell Culture, drug development REFINE Perfusion STEMCELL TECNOLOGIES Particle concentration / reactor Industries

7. Challenges Permeate Flow Operation Time Membrane fouling Pressure, flow, TMP, recirculation System configuration Interactions of parameters Scale up loopholes

8. Our Offerings Wide range of membrane selections to meet diversified applications –Materials, SA, Pore size, Geometry, etc. Systems for both R&D and productions Pre-assembled Membrane, Bags, and Tubing packages to improve your efficiencies Direct tech support from application experts

9. Spectrum Hollow Fiber Modules Broadest Range of MWCO’s (1kD through 0.65 µm) Membrane Types = PS, mPES, ME, RC, PP Small Scale = MicroKros (5 cm2) Large Scale = KrosFlo Max module (22 m2) Sterile, Non-Sterile

10. Benefits of mPES Membranes Hydrophilic membrane Low protein binding & slow fouling High selectivity High permeability Easier R & D Stable operations High performance Cost effective Characteristics Benefits

12. KRIIi makes R&D Easier Provides all necessary components for your study Automated TMP control Real time data collection and graphic analysis Small footprint Provide solid data for scale up Ideal for R&D and low volume production

13. KF Comm Data Collection System Process data exports directly into MS Excel in real time Calculated feed flow rate, shear, TMP, etc. Customizable alarm setting for process safety Pressure control with non-invasive valves Real-time graphing (virtual recording) Make the most of your experiment Recorded data for analysis

15. MBT Assemblies Permeate Flow Meter Pressure Transmitter

16. MBT Assemblies Reduce your setup or preparation time Reduce process variability Reduce your logistics burden Improve your efficiencies and productivity

17. Designed for Scalability Membrane module sizes Customizable

18. Designed for Scalability KrosFlo Research II i KrosFlo MiniKros PilotKrosFlo LDF-55 Complete line of process systems KF Comm Date Collection System generates solid data for scale up Scale up guided by technical sound rationales

19. MBT Systems for different scales Compatible with KFComm data collection systems. Customizable and optimized system setup and configuration for different systems –KR2i – Bench Scale –KMP – Pilot Scale –LDF37 – Production –Khameleon Perfusion Systems

20. Wide selection of membranes including our unique offering of high performance mPES membrane. KRIIi research TFF system make your R&D easier. Single use MBT system improve your efficiency Complete product line and common data collection software ensures smooth transfer for R&D to production. Direct onsite technical service available Competitive price SUMMARY

21. Thank You 多谢光临