1. Evidence of Evolution Exploring Various Lines of Evidence for the Theory of Evolution

2. Lines of Evidence DNA Sequences Comparative Anatomy Embryology Transitional Fossils Biogeography “Genetic Tool Kit”

3. DNA Sequences Scientists are able to isolate pieces of DNA and determine the actual sequence of nucleotides Species that are more closely related tend to have more similarities in their DNA

4. DNA Sequences Leptin = protein hormone that is important for regulating body weight and metabolism Mice without properly functioning leptin gene are morbidly obese (right) compared to normal mice (left) Leptin protein

5. DNA Sequences Compare actual sequences of DNA (leptin gene) between three different species= human, chimpanzee, mouse Predictions: how much similarity will there be? Who will be most closely related?

6. DNA Sequences First 60 nucleotides: Human: gtaggaatcg cagcgccagc ggttgcaagg taaggccccg gcgcgctcct tcctccttct Chimp: gtaggaatcg cagcgccagc ggttgcaagg taaggccccg gcgcgctcct tcctccttct Mouse: gaggga tcc ctgctccagc agctgcaagg taaggcccggggcgcgctact ttctcctcca (Mouse sequence has been shifted to line up as much as possible.) REMEMBER: Mutations can arise in the DNA sequence in a variety of forms, including nucleotide replacement, insertion, deletion, sequence inversion, etc.

7. DNA Sequences Nucleotides 121-180: Human: agtcaggagg gatgcagggc ggatggctta gttctggact atgatagctt tgtaccgagt Chimp:agtcaggagg gaggcagggc ggatggctta gttctggact atgatagctt tgtaccgagt Mouse: aggtcatgtg gacagcttgg tgttgaattc agtagttttg cagcgaggga ctctgcagac Note how the mouse sequence compares, after so many mutations have accumulated. REMEMBER: Mutations can arise in the DNA sequence in a variety of forms, including nucleotide replacement, insertion, deletion, sequence inversion, etc. (Human and Chimp sequences are identical between nucleotides 61-120.)

8. Comparative Anatomy Similarities in structures between species suggest they descended from a common ancestor. Note the color-coded bones for the limbs of these 4 mammals – though different, they share many similar bones. Describe the function of each animal’s limb on your handout.

9. http://www.eskeletons.org/ Click “Comparative Anatomy” link Compare: human, chimpanzee, and squirrel monkey Predictions: Who would be the most similar and why? What similarities and differences might you expect? Follow the directions on your handout! Comparative Anatomy

10. Embryology Ernst von Baer (1828): the more closely related any two species are, the more similar their development as embryos. In this game, you will look at pictures of embryos and guess what animal it is. Is it a snake, chicken, possum, cat, bat or human? Write your guess down on your handout, before you look at the answer!

11. Embryology Snake! Are you sure that’s your prediction?

12. Embryology Bat! Are you sure that’s your prediction?

13. Embryology Cat! Are you sure that’s your prediction?

14. Embryology Chicken! Are you sure that’s your prediction?

15. Embryology Human! Are you sure that’s your prediction?

16. Embryology Possum! Are you sure that’s your prediction?

18. Biogeography Marsupial distribution across the globe: http://evolution.berkeley.edu/evolibrary/arti cle/0_0_0/lines_11 http://evolution.berkeley.edu/evolibrary/arti cle/0_0_0/lines_11 Distribution today split on two sides of globe – how? Review a few facts of the distribution and marsupials, as well as the history of the Earth, then formulate hypothesis behind distribution Biogeography is the study of the large-scale or global pattern of distribution of species, including the history and causes of this distribution. For this activity, you will explore the history and cause behind the distribution of marsupials.

19. Biogeography Marsupial distribution across the globe: http://evolution.berkeley.edu/evolibrary/arti cle/0_0_0/lines_11 http://evolution.berkeley.edu/evolibrary/arti cle/0_0_0/lines_11 Distribution today split on two sides of globe – how? Review a few facts of the distribution and marsupials, as well as the history of the Earth, then formulate hypothesis behind distribution Marsupials are a group of mammals that give birth to live young that develop in an outer pouch of the mother. Koala Kangaroo Sugar Glider Opossum Bandicoot

20. Biogeography There is no evidence of any marsupials able to swim across the ocean. No marsupial has been observed wandering across the Asian mainland. There does not appear to be any route of migration between the two populations of marsupials. How do you think some marsupials ended up halfway across the world from the others?

21. Biogeography Continental Drift over millions of years – watch the movement of land masses

22. Biogeography Continental Drift + Distribution of Marsupials

23. Biogeography Similar reptilian Mesosaurus fossils found in both South America and Africa  evidence of continental drift (couldn’t swim the ocean, no land bridge  continents once joined)

24. Biogeography Similar reptilian Mesosaurus fossils found in both South America and Africa (couldn’t swim the ocean, no land bridge)  evidence of continental drift ( continents once joined)

25. Transitional Fossils Archaeopteryx fossils: multiple specimen found in limestone in Germany

26. Archaeopteryx: Berlin specimen

27. Archaeopteryx: Eichstatt specimen

28. Archaeopteryx: Solnhofen specimen

29. Whale Evolution Video about the transitional forms in the evolution of whales – mammals evolving from land back to sea http://www.pbs.org/wgbh/evolution/library/ 03/4/l_034_05.htmlhttp://www.pbs.org/wgbh/evolution/library/ 03/4/l_034_05.html

30. “Genetic Tool Kit” Video about Homeobox genes and implications for evolution: http://www.pbs.org/wgbh/evolution/library/ 03/4/l_034_04.htmlhttp://www.pbs.org/wgbh/evolution/library/ 03/4/l_034_04.html Answer the questions on your handout

31. Eye Evolution Video about the evolution of the eye: http://www.pbs.org/wgbh/evolution/library/ 01/1/l_011_01.htmlhttp://www.pbs.org/wgbh/evolution/library/ 01/1/l_011_01.html Answer the questions on your handout