1. The Peritoneal Vac Study Instructions for the ICU Nurse PI: Andrew Kirkpatrick pg # 02603 Derek Roberts pg# 02871 Corina Tiruta pg # 00521

2. For each patient enrolled in the study, blood and peritoneal fluid will be collected in the ICU four 4 times. Use the kits stored in the lab space in the box labeled “Peritoneal Vac Study”. Collection 1: - 24 hrs after the temporary abdominal dressing (TAC) is initiated (24 hrs after the OR procedure ends) -use the kit labelled with pt.id.001-2 for patient one, pt.id.002-2 for patient two, etc. Collection 2: - 48 hours after TAC (48 hrs after the OR procedure ends) -use the kit labelled with pt.id.001-3 for patient one, pt.id.002-3 for patient two,etc.

3. Collection 3: - day 6 after TAC (144 hours after the OR procedure ends) -use the kit labelled with pt.id.001-4 for patient one, pt.id.002-4 for patient two Collection 4: - day 26 after TAC or discharge, whichever comes first -use the kit labelled with pt.id.001-5 for patient one, pt.id.002-5 for patient two e.g. OR procedure ends at 2 am on Jan.1 -> Collection 1 will be done at 2 am on Jan.2, Collection 2 at 2 am on Jan.3, Collection 3 at 2 am on Jan. 7, Collection 4 at 2 am on Jan.28 (or hospital discharge)

4. FOR EACH TIME POINT COLLECTION: 1. COLLECT PERITONEAL FLUID: -fill-up the 4ml green cap tube labelled “PF” with peritoneal fluid. 2. COLLECT BLOOD: - 2 Green Cap 4ml tubes for plasma isolation (labeled with a “P”) - 2 Red Cap 4ml tubes for serum isolation (labeled with a “S”) fill-up the tubes to 4ml and keep them on the rack provided in an upright position 3. Fill-out the pt. gender & DOB, collection date&time on the Research Requisition 4. Send the five (4ml) tubes, the rest of the bag (containing 3 empty blue cap 15ml tubes for CLS use only) and the Research Requisition to CLS by porter

5. Fill with Blood from arterial line and keep upright in the rack STEP 2: Blood Collection: STEP 4: Calgary Lab Services: Call a porter to have the five 4mls tubes + the RR and the rest of the bag deliver to CLS STEP 1: Peritoneal Fluid Collection: STEP 3: Fill the requisition Fill-out the patient’s gender & D.O.B. and collection date and time on the research requisition (RR) PF P PSS Fill with Peritoneal Fluid