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Color Test for Specific Amino Acids
Done by :Sahar Al-Subaie
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Millon's Test Hopkins-Cole Test Sakaguchi Test
Outlines: Millon's Test Hopkins-Cole Test Sakaguchi Test
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Millon's Test
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Used for detecting the presence of monohydroxybenzene derivatives
Aim: Used for detecting the presence of monohydroxybenzene derivatives
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monohydroxybenzene derivatives:
Tyrosine, Tyrosine derivatives, Phenol
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Principle: Phenolic group of tyrosine +Millon's reagent red color (mercury salt of nitrated tyrosine)
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Millon's reagent: It is a solution of mercuric and mercurous ions in nitric and nitrous acids.
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Hopkins-Cole Test
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This test aims to detect Tryptophan (indole ring)
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Tryptophan + Hopkins-Cole H2SO4 purple ring
Principle: Tryptophan + Hopkins-Cole H2SO4 purple ring
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Sakaguchi Test
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This test is specific for the arginine (guanidine group)
Aim: This test is specific for the arginine (guanidine group)
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arginine +α-Naphthol NaOBr red color
Principle: arginine +α-Naphthol NaOBr red color
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Thank you
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