1. BioBuilder Eau d’coli BioBuilder Educational Foundation Natalie Kuldell

2. System level description (initial) Black box functions Input Output

3. System level description (initial) Black box functions Precursor compound Smell

4. Device level description (initial) Precursor compound Smell WGD: Wintergreen Generating Device WGD BGD and BGD: Banana Generating Device Device List !!

5. System level description (revised) Cell Growth Smell WGD and WGD BGD

6. Device level description (revised) Cell Growth Smell WGD: Wintergreen Generating Device WGD BGD and BGD: Banana Generating Device saGD iaGD saGD: salicylic acid Gen Dev iaGD: isoamyl alcohol Gen Dev Device List !!

7. Parts specification for stationary phase banana smell BGD cell density time “lag” “log” “stationary” OsmY is a promoter that is only active during stationary phase

8. Parts specification for log phase wintergreen smell WGD cell density time “lag” “log” “stationary” StationaryLog OsmY10 OsmY + inverter 01

9. One last modification to the E. coli chassis cell density time “lag” “log” “stationary” eliminate natural indole production saGD iaGDBGD WGD

10. “Eau d’coli” cell density time “lag” “log” “stationary” saGD iaGDBGD WGD