1. Protists I Lab 3 BIOL 171

2. Remember!: Classification System

4. Ancestral Eukaryote We’ll be looking at all of these! Protists are everywhere in Eukarya! “the junk drawer of the eukaryotes”

5. Ancestral Eukaryote We’ll be looking at all of these! Protists are everywhere in Eukarya! “the junk drawer of the eukaryotes” THIS WEEK

6. Old School: 6 Kingdoms Plants (Plantae) Animals (Animalia) Fungi (Fungi) Eubacteria Archaeabacteria Protista

8. Linnaeus [5] Linnaeus [5] (1735) 2 kingdoms Haeckel [6] Haeckel [6] (1866) 3 kingdoms Chatton [7] Chatton [7] (1925) 2 groups Copeland [8] Copeland [8] (1938) 4 kingdoms Whittaker [2] Whittaker [2] (1969) 5 kingdoms WoeseWoese [9][10] (1977,1990) 3 domains [9][10] Animalia Eukaryote Animalia Eukarya VegetabiliaPlantae Protoctista Fungi Protista (not treated) Protista ProkaryoteMonera Archaea Bacteria A constantly changing system…

9. This week.... 1 A & B: Euglenozoans Trypanosoma levisii Euglena (alive and prepared) 2 A: Stramenopiles Diatoms (living) Diatomaceous Earth Brown Algae Ectocarpus (living) Sphacelaria (living) Fucus (preserved) Laminaria (preserved) Sargassum (preserved) Water mold Saprolegnia (prepared) 2 B: Alveolates Paramecium caudatum (living) Peridinium (dinoflagellate, living) Other random dinoflagellates 2 C: Rhizarians (Foraminiferans and Radiolarians) - prepared Pro-tip: - Write these down. - Circle exercises in your manual.

10. Lab Study 1 A & B: Euglenozoans – Trypanosoma levisi (prepared slide) – Euglena (make wet mount)

11. Trypanosoma and red blood cells

12. Euglena

13. Lab Study 2 A: Stramenopiles Diatoms (Bacillariophyta) – make wet mount Also observe diatomaceous earth (the cell wall deposits from diatoms) – make wet mount and look at prepared slides

14. Diatom diversity

15. Diatom cell wall made of silica

16. Stramenopile flagella WHY do they have two? Why might they be different? Is it an adaptation? What is the function?

17. Brown Algae (Phaeophyta) Living: Ectocarpus and Sphacelaria Preserved: Fucus, Laminaria, Sargassum

18. Water mold

19. Lab Study B: Alveolates Ciliate: Paramecium caudatum – (wet mount) in manual Dinoflagellates: mixed dinoflagellates (live & wet mount), and Peridinium (wet mount) not in manual

20. Paramecium structures

22. Dinoflaggelates

23. Lab Study 2 C: Rhizarians Foraminiferans - prepared slides

24. Radiolarians - prepared slides

25. Think about… Morphological characteristics Ecology of the organism How does the organism get around? What role do they play in the ecosystem? Do they have any economic value? Where do they live? Don’t know the answer?? It’s probably a great research question! Ask me about it.