2. Lecture 7 August 15, 2005 Lehninger (4 th Edition), Chapter 16 Oxidative phosphorylation: Chapter 19

3. LNC Fig.16.13 Products from one turn of the Krebs cycle

4. LNC Fig.16.7 the only membrane- bound enzyme in the TCA cycle

5. THE GLYOXYLATE CYCLE LNC Chapter 16, pages 623-626

6. LNC Fig.16-20 Note that the figure 16-16 in the 3 rd edition has an error (there is an O instead of an H) C6C6 C6C6 C4C4 C4C4 C4C4 C2C2 C2C2 C2C2 from oxidation of fatty acids

8. LNC Fig.16.22

9. LNC Fig.16.23 isocitrate dehydrogenase: inactivated by phosphorylation isocitrate lyase: inactivated by low ATP (high AMP/ADP)

10. ELECTRON TRANSPORT AND OXIDATIVE PHOSPHORYLATION Chapter 19 in LNC

11. HEAT CO 2 HEAT CO 2 “Guinea Pig” “Charcoal”

12. “…..this fire stolen from heaven, this torch of Prometheus, does not only represent an ingenious and poetic idea, it is a faithful picture of the operations of nature, at least for animals that breathe... the torch of life lights itself at the moment the infant breathes for the first time, and it does not extinguish itself except at death.” Lavoisier, 1789

13. Carbohydrate + oxygen => carbon dioxide and water C 6 H 12 O 6 + 6 O 2 => 12 H 2 O + 6 CO 2 + ENERGY

14. RESPIRATION

15. 2

16. Wild type SDHC

18. Outer membrane Inner membrane Intermembrane space Matrix

20. LNC Fig.19.7

22. the arrangement of the complexes in the inner membrane and the order of electron flow

24. NADH

25. LNC Fig.19.15 TMPD/ascorbate CN - rotenone malonate antimycin Glutamate/malate succinate Succinate dehydrogenase membrane bound enzyme of Krebs cycle Four integral membrane protein complexes Two mobile carriers: ubiquinone and cytochrome c

26. LNC Fig.19.8

27. oxidized Coenzyme Q or Q reduced Coenzyme Q or QH 2 LNC Fig.19.2 lipid-soluble polyisoprene chain

28. Heme + apoprotein cytochrome LNC Fig.19.3

29. Structure of cytochrome c

30. LNC Fig.19.4

31. NON-HEME IRON - SULFUR CENTERS [Fe 2 -S 2 ] [Fe 4 - S 4 ]

32. LNC Fig.19.5

33. [Fe 2 -S 2 ] LNC Fig.19.5

34. [Fe 4 -S 4 ] LNC Fig.19.5

35. A bacterial ferredoxin

36. NADH O2O2

37. LNC Fig.19.9 7 or 8 NADH + Q + H +  NAD + + QH 2

38. LNC Fig.19.10 Architecture of Succinate Dehydrogenase and Reactive Oxygen Species Generation Victoria Yankovskaya,1* Rob Horsefield,2* Susanna To¨rnroth,3* Ce´sar Luna-Chavez,1,4† Hideto Miyoshi,5 Christophe Le´ger,6‡ Bernadette Byrne,2 Gary Cecchini,1,4§ So Iwata2,3,7§ SCIENCE 31 JANUARY 2003 VOL 299, p.700 www.sciencemag.org [2Fe-2S] [3Fe-4S] [4Fe-4S] succinate Succinate + Q  fumarate + QH 2

39. Complex III 8-11 polypeptides two cytochromes, b and c 1 one iron-sulfur center LNC Fig.19-11 QH 2 + 2 cyt c (Fe +3 )  Q + 2 cyt c (Fe +2 ) (ignore the protons for the moment)

40. LNC Fig.19-11b Complex III 8-11 polypeptides two cytochromes, b and c 1 one iron-sulfur center

41. LNC Fig.19-12 The Q Cycle Net equation QH 2 + 2 cyt c ox + 2H + in  Q + 2 cyt c red + 4H + out 2 Fe +3 2 Fe +2

42. Cyt c 1 red + Cyt c ox  Cyt c 1 ox + Cyt c red Fe +3 Fe +2 

43. LNC Fig19-13a Complex IV - cytochrome oxidase 9 - 13 polypeptides cytochromes a and a 3 two copper centers 4 cyt c (Fe +2 ) + O 2 + 4H+  4 cyt c (Fe +3 ) + 2 H 2 O ( and protons pumped)

45. Complex IV (schematic) LNC Fig.19-14 4 cyt c red + O 2 + 4 H + 4 cyt c ox + 2 H 2 O Fe +2 Fe +3

47. An excerpt from Table 14-7, in reverse order NADH + ½ O 2 + H +  NAD + + H 2 O  E ’ o = +0.816 v + 0.320 v = + 1.136 v  G ’ o = -n F  E’ o = - 2 x 96,480 J/V x mole x 1.136 v = - 220 kJ/mole NADH

48. End of Lecture 7 Mon, Aug. 15, 2005