1. Laboratory Safety Rules

2. While working in the science laboratory, you will have certain important _____________ that do not apply to other classrooms. You will be working with materials and apparatus which, if handled carelessly or improperly, have the potential to cause ______ or discomfort to someone else as well as yourself. responsibilities injury

3. Obey ALL Signs… CAUTION HAZARDOUS MATERIALS SATELLITE ACCUMULATION POINT UNAUTHORIZED PERSONNEL MUST KEEP OUT

4. A science laboratory can be a safe place in which to work if you, the student, are foresighted, alert, and cautious. Violating any of the following regulations will result in you being suspended from class or permanently removed from the class. The following practices will be followed:

5. 1. An _________ must be present during the performance of all laboratory work. 2. Report any _________ to the teacher immediately, no matter how_______, including reporting any burn, scratch, cut, or corrosive liquid on skin or clothing. 3. Prepare for each laboratory activity by _______ all instructions before coming to class. Follow all _________ implicitly and intelligently. Make note of any __________ in procedure given by the instructor. instructor accident minor reading directions modification

6. 4. Any science project or individually planned experiment must be __________ by the teacher. 5. Use only those materials and equipment _________ by the instructor. 6. Inform the teacher___________ of any equipment not working properly. 7. Clean up any nonhazardous _____ on the floor or workspace __________. approved authorized immediately spill immediately spill

8. Wear Your Safety Goggles! Which would you rather read? FLINN Scientific, Inc "Your Safer Source for Science Supplies" - Poster

9. I'm a little Chemist, short and stout, Here are my goggles, here are my eyes. When I don't wear my goggles, my teacher shouts, PUT THEM ON OR YOU GET OUT!

10. 8. Wear appropriate ______________, as directed by the instructor, whenever you are working in the laboratory. Safety goggles must be worn during hazardous _________ involving caustic/corrosive chemicals, heating of liquids, and other activities that may injure the eyes. eye protection activities

11. 9. Splashes and fumes from hazardous chemicals present a special danger to wearers of _____________. Therefore, students should preferably wear regular glasses (inside splash -proof goggles, when appropriate) during all class activities or purchase personal splash- proof goggles and wear them whenever exposure to chemicals or chemical fumes is possible. Students with open skin wounds on hands must wear gloves or be excused from the laboratory activity. contact lenses

12. 10. Never ______ hot equipment or dangerous chemicals through a ______ of students. 11. Check ______ and equipment instructions carefully. Be sure correct items are ______ in the proper manner. 12. Be aware if the _________ being used are hazardous. Know where the material safety data sheet (______) is and what it indicates for each of the hazardous chemicals you are using. carry group labels used chemicals MSDS

13. 16. Use a __________ pipette filler (never the mouth) when measuring or transferring small quantities of liquid with a pipette. 14. Test for odor of chemicals only by ______ your hand above the container and sniffing cautiously from a ________. 15. Eating or drinking ___ the laboratory or from laboratory equipment is ____ permitted. 13. Never ______ anything or touch chemicals with the hands, unless __________ instructed to do so. taste specifically waving distance in NOT mechanical

14. 17. When heating material in a test tube, do not _____ into the tube or point it in the direction of any person during the process. 18. Never pour ________ back into bottles, exchange stoppers of bottles, or lay stoppers on the table. 19. When diluting _____, always pour acids into ______, never the reverse. Combine the liquids slowly while stirring to distribute heat buildup throughout the mixture. look reagents acids water

15. 20. Keep _______ away from face, eyes, and clothes while using solutions, specimens, equipment, or materials in the laboratory. Wash hands as necessary and wash thoroughly at the __________ of the laboratory period. 21. To treat a burn from an acid or alkali, wash the affected area ___________ with plenty of running water. If the eye is involved, irrigate it at the eyewash station without interruption for 15 minutes. Report the incident to your instructor immediately. immediately hands conclusion

16. 22. Know the ________ of the emergency shower and eyewash station, fire blanket, fire extinguisher, fire alarm box, and exits. 23. Know the proper fire and earthquake drill ___________. 24. Roll long sleeves above the _____. Long, hanging necklaces, bulky jewelry, and excessive and bulky clothing should not be worn in the laboratory. 25. Confine long hair during a _________ activity. location procedures wrist laboratory

17. 26. Wear shoes that _____ the toes, rather than sandals, in the laboratory. cover 27. Keep work areas _____. Floors and aisles should be kept clear of equipment and materials. clean 28. Light gas ________ only as instructed by the teacher. Be sure no _______ materials (such as alcohol or acetone) are being used nearby. Use a burner with ________ caution. Keep your head and clothing away from the flame and turn it off when not in use. burners volatile extreme

18. 29. Use a fire blanket (stop, drop, and roll) to _________ any flame on a person. 30. Dispose of laboratory waste as _________ by the teacher. Use separate, designated containers (not the ___________) for the following: extinguish instructed wastebasket   Matches, litmus paper, wooden splints, __________, and so on toothpicks   _______ and waste glass Broken   Rags, paper towels, or other absorbent materials used in the cleanup of __________ solids or liquids flammable   Hazardous/toxic ______ and solids liquids

19. 31. Place books, purses, and such items in the designated storage area. Take only _________ manuals and notebooks into the _______ area. laboratory working 32. Students are not permitted in laboratory _______ rooms or teachers' workrooms without the approval of the ________ storage teacher. 33. To cut small diameter glass tubing, use a ____ or tubing cutter to make a deep scratch. Wrap the tubing in a paper towel before breaking the glass away from you with your thumbs. Fire polish all ends. file

20. 34. Hot and cold glass has the same _____ appearance. Determine whether an object is hot by bringing the back of your hand close to the object. visual 35. Match hole sizes and tubing when inserting glass tubing into a stopper. If necessary, expand the hole first by using an appropriate size cork borer. Lubricate the stopper hole and glass tubing with water or glycerin to ease insertion, using ______ to protect the hand. Carefully twist (never push) glass tubing into stopper holes. towels

21. 36. Remove all ________ glass from the work area or floor as soon as possible. Never handle broken glass with bare hands; use a counter _____ and dustpan. broken brush 37. Report broken glassware, including thermometers, to the instructor __________. immediately. 38. Operate electrical equipment only in a dry area and with _____ hands. dry 39. When removing an electrical plug from its socket, pull the ______, not the electrical cord. plug

22. 42. Students and teacher wash hands with ______ and water before leaving the laboratory area. 40. Always approach laboratory experiences in a _______ and courteous manner. serious 41. Always ______ the laboratory area before leaving. clean soap

23. 43. When heating volatile or flammable materials, use a water bath; that is, heat the materials in or over heated water, using a hot plate to heat the water. _________ all open flames. Extinguish 44. Exercise _______ in using scissors, scalpels, dissecting needles, and other sharp-edged instruments. Pass them with handles extended when handing them to other persons. caution

24. 45. Inform the teacher ___________ of any equipment not working properly. immediately 46. Use the fume hood whenever noxious, corrosive, or toxic _______ are produced or released. fumes 47. Be sure all glassware is ______ before use. Clean glassware thoroughly ______ use. Residue may cause errors in new experiments or cause a _______ reaction or explosion. clean after violent

25. The following actions will result in being dropped from the class: _______ of safety equipment including, but not limited to –eyewash stations –emergency showers –fire extinguishers –fire blanket __________ school equipment or furniture. Vandalizing Endangering Misuse __________ or causing harm to others.

26. http://lansce.lanl.gov/training/FST2004/images04/chemicals1.gif

27. LABORATORY SAFETY Chemical Hazard Label MSDS Safety Quiz Courtesy Christy Johannesson www.nisd.net/communicationsarts/pages/chem

28. Safety Symbols

29. NFPA CHEMICAL HAZARD LABEL HEALTH SPECIAL REACTIVITY (STABILITY) FLAMMABILITY

30. NFPA CHEMICAL HAZARD LABEL 2 3 4 0 Least Serious 4 Most Serious 40 Flammable vapor which burns readily Substance is stable

31. NFPA CHEMICAL HAZARD LABEL Avoid water. May detonate with heat or ignition. Severe health risk. Burns readily. Diborane 4 34 W

32. Health Health (blue) Chem. name 4 – deadly 3 – extreme danger 2 – hazardous 1 – slightly hazardous 0 – normal material Fire Hazard Fire Hazard (red) Flash Point Temp. 4 – below 73 o F Temp. 3 – 73 to 100 o F – v. flam. 2 – 101 to 200 o F – flam. 1 – over 200 o F - slightly combustible 0 – will not burn Reactivity Reactivity (yellow) 4 – may detonate 3 – shock or heat may detonate 2 – violent chem. reaction 1 – unstable if heated 0 – stable Specific Hazard OXY – oxidizer ACID – acid ALK – alkali COR – corrosive W – use no water RAD – radiation hazard NFPA Label

33. NFPA CHEMICAL HAZARD LABEL Complete Label for Acetone

34. NFPA CHEMICAL HAZARD LABEL Complete Label for Phosphine

35. MSDS Material Safety Data Sheet On file for all purchased chemicals. Includes all information shown on a chemical label and more. Different formats are used by different chemical companies.

36. MSDS

37. Health Hazard 4 Very short exposure could cause death or serious residual injury even though prompt medical attention was given. 3 Short exposure could cause serious temporary or residual injury even though prompt medical attention was given. 2 Intense or continued exposure could cause temporary incapacitation or possible residual injury unless prompt medical attention is given. 1 Exposure could cause irritation but only minor residual injury even if no treatment is given.irritation 0 Exposure under fire conditions would offer no hazard beyond that of ordinary combustible materials.combustible

38. FLAMMABILITY 4 Will rapidly or completely vaporize at normal pressure and temperature, or is readily dispersed in air and will burn readily.normal pressure and temperatureair 3 Liquids and solids that can be ignited under almost all ambient conditions. 2 Must be moderately heated or exposed to relatively high temperature before ignition can occur. 1 Must be preheated before ignition can occur. 0 Materials that will not burn.

39. INSTABILITY 1 1 4 Readily capable of detonation or of explosive decomposition or reaction at normal temperatures and pressures.explosivedecompositionnormal temperatures and pressures 3 Capable of detonation or explosive reaction, but requires a strong initiating source or must be heated under confinement before initiation, or reacts explosively with water.explosivereacts explosively with water 2 Normally unstable and readily undergo violent decomposition but do not detonate. Also: may react violently with water or may form potentially explosive mixtures with water.decompositionreact violently with waterexplosivemixtures 1 Normally stable, but can become unstable at elevated temperatures and pressures or may react with water with some release of energy, but not violently.react with water with some release of energy 0 Normally stable, even under fire exposure conditions, and are not reactive with water. 1 Prior to 1996, this section was titled "Reactivity". The name was changed because many people did not understand the distinction between a "reactive hazard" and the "chemical reactivity" of the material. The numeric ratings and their meanings remain unchanged.

40. OX This denotes an oxidizer, a chemical which can greatly increase the rate of combustion/fire.oxidizerchemicalcombustion Unusual reactivity with waterUnusual reactivity with water. This indicates a potential hazard using water to fight a fire involving this material. This section is used to denote special hazards. There are only two NFPA 704 approved symbols

41. ACID This indicates that the material is an acid, a corrosive material that has a pH lower than 7.0acidcorrosive materialpH ALK This denotes an alkaline material, also called a base. These caustic materials have a pH greater than 7.0basepH This denotes a material that is corrosive (it could be either an acid or a base).corrosive The skull and crossbones is used to denote a poison or highly toxic material. See also: CHIP Danger symbols.poisonhighly toxicCHIP Danger symbols The international symbol for radioactivity is used to denote radioactive hazards; radioactive materials are extremely hazardous when inhaled.inhaled Indicates an explosive material. This symbol is somewhat redundant because explosives are easily recognized by their Instability Rating.explosive Instability Rating