1. Histology
Muscle Lab
Stephen Ernst, Ph.D. / Matt Velkey, Ph.D.

2. 58 Skeletal Muscle Longitudinal Striated Muscle Fiber (cell)
Endomysium
Peripheral Flattened Nuclei
Striations

3. 2 consecutive Z line = Sarcomere
58L Skeletal Muscle
Dark A Band (myosin)
Light I Bands
(actin)
Divided by Z Line
2 consecutive Z line = Sarcomere

4. Slide 58 Skeletal Muscle Cross Section
Peripheral Nuclei
Muscle Fibers

5. Slide 59 H&E Skeletal Muscle Peripheral Nerve
(Use this slide to examine CT layers in skeletal muscle; ignore,
nerve fasicles which are also present)
Skeletal Muscle
Peripheral Nerve

6. Slide 59 Skeletal Muscle Cross Section H&E Stain
Individual Muscles
Tendon
Epimysium
Perimysium
SKIN:
Epimysium
Epidermis
Adipose
Tissue
Dermis

7. Slide 59 Trichrome Stain
Endomysium
Perimysium

8. Slide 71 (even)
Axons
Muscle fibers (purple)
Neuromuscular
junctions

9. Slide 71 (even)
Neuromuscular Junctions
(a.k.a. motor end plates)

10. Slide 71 (even)
Axon
Node of Ranvier
Neuromuscular junction

11. Slide 71 (even)
Axon
Neuromuscular junction
(viewed from top)

12. Slide 71 (even)
Axon
Neuromuscular junction
(viewed from top)

13. Skeletal muscle
Neuromuscular spindle
Slide 71 (odd)

14. Slide 71 (odd) outer capsule of neuromuscular spindle inner capsule
Intrafusal fibers
Skeletal muscle (extrafusal fibers)

15. NOT a neuromusclar spindle!
Slide 71 (odd)
Skeletal muscle
(extrafusal fibers)
Peripheral nerve
NOT a neuromusclar spindle!

16. 57 Cardiac Muscle Longitudinal Fibers Cross Sectioned Fibers
(Note: Slide 98 is also quite
good for Intercalated disks)
Longitudinal Fibers
Cross Sectioned Fibers
branching
Intercalated
Disk
Large Nuclei
centrally placed

17. 29 Smooth Muscle (Intestine)
Cross Section
Loose Connective Tissue
Small,Fusiform (spindle shaped)Fiber
Central Flattened Nucleus
Longitudinal Section

18. Cross Section Smooth Muscle Fasicle
250 Vagina Smooth Muscle
Cross Section Smooth Muscle Fasicle CT CT
Longitudinal Smooth Muscle Fasicle CT

19. 250 High Power Smooth Muscle FascilesCT