1. Institute of Laboratory Animal Science University of Zurich The Assessment of Pain in Mice using Telemetry M. Arras

2. Institute of Laboratory Animal Science University of Zurich Pain? …temporarily (2 weeks) paralyzed hind legs caused by activated transgene

3. Institute of Laboratory Animal Science University of Zurich Pain? Answers 4sacrifice 9pain therapy 11severe pain 16intermediate pain 12no pain

4. Institute of Laboratory Animal Science University of Zurich Goal of the study Objective measurements of pain and suffering in laboratory mice

5. Institute of Laboratory Animal Science University of Zurich measurements: 30 sec/5 min Telemetry

6. Institute of Laboratory Animal Science University of Zurich Model intermediate pain transmitter implantation mild pain vasectomy no pain recovery

7. Institute of Laboratory Animal Science University of Zurich Parameters What we measure –heart rate (ECG) –body temperature –activity How we interpret –circadian rhythm

8. Institute of Laboratory Animal Science University of Zurich mild pain 1day chronic tumour disease 1day intermediate pain 1da y normal Heart Rate 1day

9. Institute of Laboratory Animal Science University of Zurich Body Temp. normal 1day chronic tumour disease 1da y intermediate pain 1da y mild pain 1da y

10. Institute of Laboratory Animal Science University of Zurich intermediate pain 1da y Activity normal 1day mild pain 1da y chronic tumour disease 1da y

11. Institute of Laboratory Animal Science University of Zurich Indicators of pain PHYSIOLOGY heart rate body temperature GENE REGULATION identification of genes related to pain and stress in transgenic mice BEHAVIOR activity behavioral tests score sheet