1. NATIONAL PROGRAM 302: PLANT BIOLOGICAL AND MOLECULAR PROCESSES THE LAST 5 YEARS Leon Kochian, Don Ort and Carroll Vance

2. Our analysis will set the stage for the presentation by the external peer review team What is National Program 302? How was the self analysis report generated? Overview of NP302 research over the last 5 years What about the next 5 years?

3. National Program 302 is Unique Within ARS Mission is to conduct fundamental research on plant processes. This program does not focus on specific crops/traits, although research on both model plant and many crop species is conducted. This information is used by scientists in this and other national programs for crop improvement with regards to: Crop productivity and efficiency Crop quality and safety Improved tolerance to biotic and abiotic stresses Improved environmental quality Thus NP302 cross-cuts other national programs with regards to their problem solving missions, and is based on an integrative approach across all levels of biological organization (from genes to the crop in the field)

4. NP 302 PROGRAM COMPONENTS Component I. Analysis and Modification of Plant Genomes Molecular Characterization of Plant Genetic Systems Plant Transformation Systems and Influence of Transgenes on Genome Structure and Function Component II. Biological Processes That Determine Plant Productivity and Quality Plant Productivity and Efficiency of Resource Use Plant Tolerance to Environmental Stresses Biological Basis for Expression of Value-added Traits Component III. Mechanisms of Plant Interactions with Other Organisms Crop Plant Interactions with Beneficial Microorganisms Biological Interactions that Impact Food Safety, and Secondary Plant Products Biological Interactions that Reduce Environmental Pollution

5. The Challenge for 21 st Century Agriculture Year1940197020002030 Population2.3 billion3.7 billion6.1 billion8.1 billion Area in production 990 m ha1450 m ha3600 m ha 4900 m ha Average yield 1.15 t/ha1.7 t/ha3.8 t/ha >>5.05 t/ha >3.8 t/ha The major challenge is to increase both crop yields and the land area under production. This will mean getting more from less – sustainably increasing yields on marginal soils

6. Crop Genomics and Proteomics (Tool Development) Mechanisms Regulating Photosynthesis Shoot and Root Development Biotic and Abiotic Stress Germplasm Improvement Plant Metabolism Flowering Biotechnology Risk Assessment NP 302 ADDRESSES THE FUNDAMENTAL BIOLOGY OF CRITICAL ISSUES IN AGRICULTURE

7. The Process: How Was the Self Assessment Generated? Questionnaire was sent out to all lead scientists of CRIS projects for which 302 was a major or minor component Scientists provided information on: Research focus for their CRIS Research accomplishments (in the context of the “big picture”) Quantitative data relating to research progress Issues to be addressed for next five year Action Plan CRIS annual reports were also used

8. Functional Genomics EST Sequencing Transformation Promoter Identification Micro (Macro) Arrays Proteomics Tilling DEVELOPMENT OF FUNCTIONAL AND COMPARATIVE GENOMICS RESOURCES Comparative Genomics Integrated Genetic/Physical Maps SNP Development Development of other Mapping Resources Computational Biology

9. BIOTECHNOLOGY RISK ASSESSMENT Chloroplast transformation to limit transgene drift Genes to reduce pollen fertility in GE plants Non-antibiotic resistant selectable markers Site specific transformation systems for more precise DNA insertion Constructs for multiple (stacked) gene insertion

10. BIOCHEMISTRY/PHYSIOLOGY OF PLANT FUNCTION Plant development Photosynthesis Carbon assimilation Secondary plant metabolism Enhanced crop quality Signal transduction

11. PLANT FUNCTION: ACCOMPLISHMENTS Molecular/biochemical regulation of C and N assimilation Signaling between pollen and pistil (regulation of pollen fertility and hybrid production) Molecular regulation of lignin synthesis Increased S-amino acids in soy protein Hypo-allergenic products soybean protein guayule latex Increased  -carotene, lycopene & other carotenoids Neutraceuticals (i.e. resveratrol)

12. ABIOTIC AND BIOTIC INTERACTIONS Plant interactions with beneficial organisms: rhizobia, mycorrhizae and endophytes Plant disease resistance Plant interactions with pests Mineral nutrient uptake and tolerance to metal stresses in the soil Tolerance to temperature extremes and drought

13. BIOTIC INTERACTIONS: ACCOMPLISHMENTS Gene expression profiling has identified novel root nodule specific genes Knockouts for root nodule genes developed in legumes Resistance genes for SCN and hessian fly identified Reduction of mycotoxins in food crops Breeding (both traditional and molecular) for resistance to toxin producing fungi Mode of action & genes to reduce Fusarium toxins Metabolite screening in resistant crops to identify plant compounds that inhibit fungal growth & mycotoxin production

14. ABIOTIC INTERACTIONS: ACCOMPLISHMENTS Crop tolerance to high temperature Rubisco activase & other enzymes Restored cell function after damage Assays for ‘heat tolerance’ genes Improved water-use efficiency Genes that enhance plant survival to freezing temperatures Genes and mechanisms of plant tolerance to toxic metals (heavy metals and aluminum) Genetic and physiological basis for mineral nutrient efficiency

15. IMPACT AND LEVERAGE ARS scientists in NP 302 are proactive in expanding and leveraging their resources Actively competing for and securing NSF, NRI, DOE, BARD grants Training of PhD students and postdoctoral associates Indicators of impact Publications in high profile journals Extramural grant funding Service on editorial boards, scientific societies, and extramural grant panels

16. THE NEXT FIVE YEARS What emergent or re-emergent areas need to be addressed within the new NP 302 Action Plan? What strategies will best ensure access of ARS scientists to enabling technologies as they become available? What are the best strategies and tools to help promote integration of interdisciplinary research both within NP 302 and between 302 and other national programs?