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Normal Flora Microbiotica
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Synder Deep CH2O fermentation –Glucose Differential –Rate of change –susceptibility Selective for lactobacillus –pH = 4.8 –Indicator = bromcresol green –Dental caries
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BAP Divide Plate Swab throat Differential –Sheep Blood –Hemolysis Hemolysin exotoxins Types –Gamma –Alpha –Beta
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Chocolate Agar Divide Plate Swab throat Differential –Growth –Oxidase test Neisseria Lysed RBCs
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MSA Selective Differential Divide plate Swab Skin –Nose –Face Results –Fermentation –acid end products –yellow Components –7.5% NaCl –mannitol –pH indicator = phenol red
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MH-T Divide plate Swab skin –Ears –Palms Differential –Diptheroids Tellurite –Reduced to tellurium –Black (grey) colonies
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Nutrient Agar TSA –Not selective –Not differential Divide plate Swab environment –Moisten swab –Label source
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Sabourauds Selective –Yeast –Fungus +/- Differential Acidic pH Divide plate Swab environment –Moisten swab –Label source
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Urine Culture TSA plate Calibrated loop (0.01ml) Clean catch sample Swab plate –Confluent –Isolation Calculate CFU/ml
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Normal Flora Results
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Snyder Deep Results CH2O fermentation –Glucose Differential –Rate of change –Susceptibility for caries Selective for lactobacillus –pH = 4.8 –Indicator = bromcresol green –Green Yellow –Dental caries Slight Mild Moderate Severe
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BAP results Not selective Differential Hemolysis –Hemolysin exotoxins –Types Gamma Alpha Beta
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Chocolate Agar Results Differential –Growth –Lysed RBCs –CO2 = capnophiles Oxidase Test –Neisseria
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Oxidase Test Oxidase Reagent –Cytochrome C in ETC –Dark Purple = positive –Neisseria
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Oxidase Reaction
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MSA Results Selective Differential Results –Fermentation –acid end products –yellow Components –7.5% NaCl –Mannitol –pH indicator = phenol red
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MH-T Results Differential –Diptheroids Mueller Hinton –Nutrient agar –Controlled proteins/ions –Diffusion Tellurite –Reduced to tellurium –Black (grey) colonies
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Sabarouds Results Selective –Yeast –Fungus +/- Differential Acidic pH –5.5 –acidophiles
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TSA Environmental Culture
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UTI CFU/ml # colonies/0.01ml UTI –= > 1x10 (5)/ ml –Clinical signs –Other urine tests
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