1. Microbial Diversity Project leader: Daniela Goltsman
Jose Villanueva, Victoria Partida,
Katherine Cantarero

2. Table Of Contents. . . Introduction Inoculation Isolation FISH Results
Conclusion

3. Background Why are microorganisms important?
How are microorganisms studied today?
Aseptic technique
Dyes
What was the goal of our experiment?
To study microbial diversity around our environment

4. Diversity of life
Tree and 16s

5. Sampling Sites Our samples: Why did we choose these samples?
Jose’s bathroom
UC Berkeley’s Strawberry Creek
Foothill Rec. Room Keyboard
Katherine’s Dorm
Why did we choose these samples?

6. Which location do YOU think is the diverse or “dirtiest” ?

7. What is inoculation?
The introduction of microorganisms into any media.
For us this process took about three days to complete.
We incubated at C

8. Jose’s Bathroom

9. Katherine’s dorm

10. Foothill Rec. room keyboard

11. UC Berkeley’s Strawberry Creek

12. Isolation
Taking one colony or “population” of one type of microorganism and making those populations reproduce and grow.
To our eye.

13. Isolate from Jose’s bathroom

14. Isolate from rec. room keyboard

15. UC Berkeley’s Strawberry Creek

16. What is FISH? FISH: Fluorescent In Situ Hybridization.
This is a powerful technique that can be used to evaluate and analyze the presence of organisms in their community, their phylogeny, morphology, and number by targeting the 16S rRNA.

17. FISH probes What is a probe? Our dyes:
Short sequence of DNA that hybridizes (binds) to a segment of the 16S rRNA.
Each probe carries a different color dye.
Our dyes:
4 different probes 3 different colors
Blue- Gama proteobacteria & Eukaryotic cells
Green- Universal bacteria
Red- Beta proteobacteria

18. Sample 1 LAPTOP. Green: bacteria, red: beta-proteobacteria.

19. Sample 2: STRAWBERRY CREEK Green: universal bacteria, red: beta-proteobacteria, soft blue: gama-proteobacteria, bright blue: eukaryotes.

20. Sample 3:KATHERINE’S ROOM Red: beta-proteobacteria, blue: eukaryotes.

21. Sample 4. Green: universal bacteria, blue: eukaryotes

22. Conclusion In conclusion, we learned that:
Microorganisms are EVERYWHERE!
There are many kinds of bacteria that take on many shapes and sizes.
Keyboards and bathrooms are the most diverse

23. Thank you!