1. Genomics
Chapter 18

2. So what is Genomics?
Genetic Maps vs Physical Maps

3. Generation of Physical Maps
Restriction Maps
Chromosome-banding patterns
Radiation Hybrid maps

4. STS
Only occur once in the genome
Can be used to make physical maps

5. Whole Genome Sequencing
Needs to be able to sequence larger molecular clones….how can they do this?
Clone-by-clone vs shotgun

6. Human Genome Project
“Completed” in 2000….with 341 gaps still present in 2004
Found only around 25,000 genes
How do we compare to fruit flies…what about rice?

7. The BLAST algorithm
Bioinformatics

8. Protein-encoding DNA in Eukaryotes
Single-copy genes
Segmental Duplications
Multigene Families
Tandem Clusters

9. Non-coding DNA in eukaryotes

10. So How can we tell what is actually expressed?
Expressed Sequence Tags

11. So are we all the same?
SNP’s
Linkage disequilibrium
halotypes

12. Comparative Genomics and Proteomics
Look at similarities and differences between species based on genomes
Synteny
Functional Genomics
DNA Microarrays

13. DNA Microarrays
Gives the ability to observe and manipulate genes to see which ones are functional where

14. Tansgenics
Can be used to determine if two genes from different species have similar functions in other species

15. Applications of Genomics
Identify Infectious Diseases
Help Improve Crops

16. Ethical Issues of Genomics???
Think Gataca