1. ACADEMIA SINICA IBMS 嚴仲陽 美國德州貝勒醫學院 博士 Specialty:
Molecular & Cell Biology Hematopoiesis Apoptosis Regulation

2. Method Yeast Two-hybrid

3. Material Yeast strain：L40 (-his、-trp、-leu、-ade)
Plasmid：pBTM116 ＆ pACT2
Competent Cell：HB101
Yeast DNA purification kit
E coli. DNA purification kit

4. Result
No Result ……

5. Normal Transcription

6. Yeast Two-hybrid Transcription

7. Plasmid construction

8. Bait ＆ Prey

9. Bait－pBTM116

10. Prey－pACT2

11. Yeast Two-Hybrid

12. Transformation ＆ Assay -Trp, -Leu, -His

13. β-galactosidase assay

14. gs3955 ＆ library Yeast Transformation Pick colonies
Selection three times
β-galactosidase assay
Yeast DNA purification
Transform to HB101
Bgl Ⅱ check
Retransform to yeast
Sequencing

15. My summer vacation
Experiment
Travel
Camp