1. (classical) genetics
causality
DNA
phenotype
‘reverse’ genetics

5. T C

14. The ‘gapped duplex’ method of in vitro mutagenesis

15. Gapped duplex method with phenotypic selection

22. Target : replace Trp by Phe Screening aid : MnlI cleavage
Mismatch primer :
…GCCCTGGGCTTCGGTGGCA…
Creating or removing a restriction recognition site as an aid in screening for site-specific mutants

23. Codewoord tabel voor ‘reverse translation’
Leu = 1T Arg = 3G Ser = 567