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Welcome to the continuation of Biol 213 Genetics! What’s in the mail? EditBase - DNA processing program Mystery sequence Problem Set 3 now available Lab.

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Presentation on theme: "Welcome to the continuation of Biol 213 Genetics! What’s in the mail? EditBase - DNA processing program Mystery sequence Problem Set 3 now available Lab."— Presentation transcript:

1 Welcome to the continuation of Biol 213 Genetics! What’s in the mail? EditBase - DNA processing program Mystery sequence Problem Set 3 now available Lab 2 - long Genetics News Feedback welcome

2 Upcoming events Monday, Sept 18: Problem day (Problem Set 3) Friday, Sept 22: Problem day (Problem Set 4) Monday, Sept 25: First exam (7:00 PM) Monday, Sept 25: in class….?

3 Today’s events DNA Replication Integration of leading/lagging strand synthesis Inborn errors of metabolism How does alkaptonuria work Beadle & Tatum’s experiment with Neurospora How does alkaptonuria work

4 DNA Replication How can DNA polymerase go left to right and right to left simultaneously?

5 Relationship between genes and proteins One gene - many protein gene RNA Protein precursor

6 Relationship between genes and proteins One gene - many protein Protein precursor lactose maltose Lactose degrading enzymeMaltose degrading enzyme

7 Relationship between genes and proteins Many genes - many protein

8 Relationship between genes and proteins Many genes - many protein One gene - One protein

9 Phenylalanine degradation Dietary protein phenylalanine other amino acids tyrosine p-hydroxyphenylpyruvic acid homogentisic acid maleylacetoacetic acid Homogentisic acid oxidase

10 Phenylalanine degradation Dietary protein phenylalanine other amino acids tyrosine p-hydroxyphenylpyruvic acid homogentisic acid maleylacetoacetic acid Homogentisic acid oxidase Homogentisic acid Black oxidation product Problems Alkaptonuria

11 Phenylalanine degradation Dietary protein phenylalanine other amino acids tyrosine p-hydroxyphenylpyruvic acid homogentisic acid maleylacetoacetic acid Phenylalanine hydroxylase Phenylketones (neurotoxins)

12 Life cycle of yeast

13 How to get mutants in Neurospora Haploid Neurospora radiation Auxotroph? Diploid Neurospora radiation No auxotrophs screen 1000’s

14 How to get mutants in Neurospora self cross 1000’s auxotroph? Could you do this with humans? back cross 1000’s self cross mixed mating types

15 Analysis of Beadle & Tatum Expt 1. One gene - many enzymes 2. Many genes - one or more enzymes 3. One gene - one enzyme

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