1. Piama birth cohort Gerard H. Koppelman Pediatric Pulmonology and Pediatric Allergology Beatrix Children’s Hospital, University Medical Center Groningen, the Netherlands Groningen Research Institute for Asthma and COPD

2. Piama: part of the Allergenic study A nation wide Dutch collaborative study on atopy development in three birth cohorts Piama Prevask KOALA

3. The ALLERGENIC study PIAMA PREVASC KOALA Bottema et al, Eur Respir J 2008; 32: 593–602

4. ALLERGENIC

5. 03 mnd1 jr4 jr6 jr PR 8 jr2 jr Spec IgE: N=1732 Total IgE: N=1899 Spec IgE: N= 1206 Total IgE: N=1242 Spec IgE: N=1093 Total IgE: N=1093 IgE in Allergenic Spec IgE: N=212 Total IgE: N=212 Spec IgE: N=714 Total IgE: N=748

6. IL13 / 3’UTR and serum IgE Bottema et al, Eur Respir J 2008

7. PIAMA study 1) general overview 2) what are the strengths 3) what resources do you have 4) what areas would you be interested in collaborating on?

8. PIAMA study - overview a placebo controlled intervention study in which the effect of mite impermeable mattress covers was studied -a natural history study -Recruitment took place in 1996-1997 -Total n = 4,146

9. 01 jr4 jr6 jr8 jr2 jr Measurements in Piama Clinic visit: -Total IgE -Sp IgE -FEV1 -BHR -NO Clinic visit: -Total IgE -Sp IgE -FEV1 -BHR -NO House visit: -Exposure -LPS -Der P1 -Eczema Cord blood IgE

10. PIAMA study - age 4 -All 1,327 children of allergic mothers -a random sample of 663 children of non- allergic mothers -Medical examination: 1,288 children -DNA was collected from 1,046 of these children

11. PIAMA study: strenghts Excellent follow up, now untill age 11 Homogeneous Dutch population Potential to pool data with 2 other Dutch birth cohorts Environmental data: LPS, Der P1 in subset Air pollution – based on address information

12. CD14 -550, dog and IgE Dog Age (years) Bottema et al, Eur Respir J 2008; 32: 593–602

13. PIAMA study: resources DNA is now available of over 2,000 children GWAS data in n = 400 (GABRIEL) DNA: genomic / amplified DNA Follow up investigations ongoing

14. PIAMA study: collaboration Field of interests: –Phenotype: Bronchial hyperresponsiveness / lung function / NO Early wheezing phenotypes –G x E Passive smoking, intervention study Air pollution Pooling of data: LPS, Der P1 –Open towards collaborations

15. Acknowledgement UMCG / University Groningen: N.E. Reijmerink R.W.B Bottema D.S. Postma M. Kerkhof J. Gerritsen Erasmus MC Rotterdam: J. de Jongste IRAS, Utrecht: B. Brunekreef RIVM, Bilthoven: H.A. Smit University Hospital / University Maastricht C.P. van Schayck F. Stelma C. Thijs