1. Genome Sequence Analysis of Chlamydia trachomatis Mopn_tet_14 Anjali Menon Rockey Lab Dryden Hall, OSU

2. Chlamydia

3. Objectives Part 1 ▫Grow and amplify mopn_tet_14 ▫Send to the Illumina machine for sequencing

4. Objectives Part 2 ▫Align gene sequences using MUSCLE, a multiple sequence alignment algorithm ▫Use Perl programming language to create a comparative database for known chlamydial genomes

5. Methods and Materials ▫Glycerol stock of mopn_tet_14 ▫PCR ▫DNA Gels ▫Illumina Sequencing ▫Perl and VIM programming languages to create database for comparative analysis

6. Process

9. Future Projects

10. Summary ▫Grew and amplified mopn_tet_14 ▫Sequenced, identified regions of uncertainty ▫Designed primers to cut individual regions ▫Confirmed mopn_tet_14 sequence ▫Used MUSCLE to align gene sequences ▫Started using Perl and VIM to organize sequences, and ultimately, to create a comparative database for completed chlamydial genomes

11. Acknowledgements ▫HHMI ▫Kevin Ahern ▫OSU’s CGRB ▫Dr. Dan Rockey ▫Brendan Jeffrey ▫Kelsi Sandoz