2.  94 degrees C 30 seconds  64 degrees C 30 seconds  72 degrees C 1 minute  After 25 cycles finish with 72 degrees C for 5 minutes Novablue cells were used

3. Our PCR Product Molecular weight marker

4. Our vector after purification, it’s pretty weak but it’s there.

5. No colonies were formed after ligation. Therefore a backup culture made by Lasse was used for screening and analysis. Novablue cells were used

6. Reference PCR fragment from another group Ligated vector, pretransformation. No vector is visible. Screening of Lasses colonies, you can clearly see the result when comparing our leftmost sample with the PCR reference.