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Chymotrypsin Proteases ClassNucleophile(s)Base Covalent Intermediate Serine protease Cysteine protease Aspartyl protease Metalloprotease (Zn) Ser/water.

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Presentation on theme: "Chymotrypsin Proteases ClassNucleophile(s)Base Covalent Intermediate Serine protease Cysteine protease Aspartyl protease Metalloprotease (Zn) Ser/water."— Presentation transcript:

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2 Chymotrypsin

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4 Proteases ClassNucleophile(s)Base Covalent Intermediate Serine protease Cysteine protease Aspartyl protease Metalloprotease (Zn) Ser/water Cys/water water His Asp Glu Yes No

5 Proton Shuttle in Carbonic Anhydrase

6 RNA Secondary and Tertiary Structure Hepatitis Delta Virus Ribozyme

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8 Hydrolysis of a phosphodiester bond by a Ribozyme

9 Clone and test for Ribozyme that Hydrolyzes ATP (RT-PCR) Genetic diversity More genetic diversity! in vitro Evolution of a Ribozyme (affinity chromatography)

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