1. Pegasus MSF SatElysium Launch Readiness Review Jordan Burns, Brenden Hogan, Miranda Link, Cody Spiker, Chris Dehoyos, Hemal Semwal November 1 st, 2011

2. Mission Overview Mission Statement: “To test the effects of high altitude flight upon a closed container containing Streptococcus mutans bacterium, and recording their response to discover if harsh high altitude effects bacterial reproduction and survival.” Why? To test the validity the of the recorded results of the 1967 flight of Surveyor III.

3. Design Overview 21x21x12 cm 2 levels 6 different samples, 3 locations with varying conditions Motorized door 4 sensors (2) Temperature Pressure Visible Light

4. Hardware/Software Testing Software / Hardware Testing No major problems occurred with our software, besides getting everything to run right. FIX: Trial and Error testing and corrections Cooler Test Data collected from all sensors-compared to control data EXCEPT HOBO Malfunction: only programmed to output voltage readings, not temperature and humidity

5. Average: 13 No Light: 0 Absolute Light: 1024

6. Close Door Open Door Average: 33.5 degrees Celsius

7. Open DoorClose Door Average: 25.3 degrees Celsius Cooler Testing

8. Open Door Close Door Average: 83788.67 Pascals Cooler Testing

9. Incubation Testing Each test has been overlapped in order to isolate each potential problem that may occur. Multiple tests show the desired traits from a healthy culture are: Translucent, White Colonies Expansion Growth Colony size larger than 1 mm After analysis of S. mutans, it was discovered that this strand is non-aerobic and a vacuum test would be inconclusive towards any results.

10. This is the Desired Culture

11. Flight Samples-To be tested The actual flight cultures are going to be one day shy of full maturity. This means that the actual cultures for flight will be grown on the Thursday before launch day, November 4, 2011. Being slightly immature, these cultures will be at their most vulnerable point of outside factors affecting their growth. Being exposed at this point means that after flight and exposure, any change in the bacteria will be the most noticeable.

12. Structural Testing Drop/ Roll Test Our drop test revealed that our structure was NOT SOUND The slits for our shelves were too deep Our tape was not secure FIX: Pre-cut BASES to slide the shelves in Cleaner and more precise cuts were made, and more efficient gluing. Whip Test revealed that the solid faces of our structure could not support the pulling of the tether. FIX: A wall that runs across the middle of the CubeSat to give the wall support

13. Expected Results We expect to find that the bacteria will be resilient enough to survive both the extreme radiation and temperature environments. The samples will grow normally after the flight

14. Worries 1.Science Failure: The motorized door will not open. No light radiation will be let into satellite environment. 2.Structural Failure: The motorized door will rip off during burst

15. Requirements Matrix RequirementsDescriptionOrigin AConstruct a BalloonSat that shall survive an ascent to 30 km above the surface of Earth and the following decent while maintaining complete functionality. Mission Statement BWeight shall not exceed 850 grams, nor a budget of $370 ($250 + $20/ea out of pocket). CSatElysium shall safely transport 6 samples of streptococcus mutans during its flight, to later study the effects of temperature and radiation in the stratosphere on the bacteria. DStreptococcus mutans samples shall be recovered and analyzed post-flight. ESatElysium shall carry a camera payload to document footage of the flight of the exterior of the satellite.