1. Autoclave By: Carolina Gallango-Brun Sana Khan Vedrana Sahovic

2. Definition Autoclave is a pressurized device designed to heat aqueous solutions above their boiling point at normal atmospheric pressure to achieve sterilization Fig. 1. Autoclave machine

3. Brief History 1450 B.C.E. Moses 460-377 B.C.E. Hippocrates Lazzaro Spallanzani Pasteur Denis Papin Fig. 2. Papin’s steam cooker

4. 20 th Century It was finalized by Charles Chamberland in 1987 Colleague of Pasteur Building up on Papin’s marmite From culinary to medicine Fig. 3. Charles Chamberland 1851-1908

5. How Des it Works? Liquid H 2 O cannot be heated above 100°C in an open vessel At 100°C boils H 2 O heated in a sealed vessel Pressure rises Boiling point of H 2 O is raised

6. The Process Air removal Downward displacement Steam pulsing Vacuums pumps Superatmospheric Subatmospheric

7. Autoclave Usage Microbiology Medicine Body piercing Veterinary science Dentistry Podiatry Metallurgy …

8. Video Clip http://youtu.be/4zFDeN1pLro http://youtu.be/4zFDeN1pLro

9. References http://en.wikipedia.org/wiki/Autoclave http://138.23.152.128/protocols/autoclave%20operation.pdf