1. Recombinant DNA technology DNA isolated from two sources and cut by the same enzyme Gene of interest Recombinant DNA Plasmid (vector) Transgenic organisms Useful products Gene for pest resistance Gene for toxic-cleanup bacteria Some uses of proteins: Protein for “stone-washing” jeans Protein for dissolving clots Some uses of genes:

2. Restriction Endonucleases Recombinant DNA technology

3. First DNA Cloning Experiment Cohen et al., 1973 “Recombinant DNA” Recombinant DNA technology

4. Cloning Vectors: pBR322 plasmid Recombinant DNA technology

5. Genetically Modified (GM) Foods In the United States today, roughly one-half of the corn crop and over three-quarters of the soybean and cotton crops are genetically modified. ●Corn has been genetically modified to resist insect infestation.

6. Golden Rice Genetically Modified (GM) Foods

7. Palmiter et al. (1982) Dramatic growth of mice that develop from eggs microinjected with metallothionine-growth hormone fusion genes. Nature 300: 611-615 “Supermice” Recombinant DNA technology

8. Weeding Out Land Mines First Glowing Pet Recombinant DNA technology

9. Molecular Techniques Nucleic Acid Extraction Buffer Components: SDS (sodium dodecyl sulfate) NaCl Sodium citrate EDTA Polymerase Chain Reaction