1. Cloud Computing at Amazon’s EC2 Joe Steele jrsteele@unomaha.edu

2. Grid Computing Shared resources – many computer clusters transferring data and running jobs. Geographically distributed. Cross-grid collaboration. Idea is analogous to electric power network (grid), where power generators are distributed, but users access electric power without bothering about the source of energy and its location.

3. LHC Computing Grid (LCG)

4. Cloud Computing What if I don’t have my own cluster? Cloud computing refers to a cluster that invites users to send jobs. (SaaS –Software as a Service) Computation, software, data access, and storage services that do not require user knowledge of the location or configuration of the system. Term comes from the cloud drawing used in the past to represent the telephone network, later represents the internet.

5. Cloud Computing

6. Private companies large data centers. When considering operational costs, 50k servers are cheaper per cpu then 1k servers (5 to 7 times cheaper). Amazon: $0.085/cpu-hour No minimum, maximum No contract

7. Amazon E2 aws.amazon.com Computing cluster – create an account and provide a credit card. Let Amazon take care of the hardware.

8. Cloud BioLinux JCVI (J. Craig Venter Institute) created cloud version of NERC BioLinux VM. An Ubuntu machine with over 100 NEBC software packages. Image stored at EC2, is available to be copied at no charge, by EC2 users.

9. http://aws.amazon.com

10. Create a new account

11. Enter your information

12. Sign up for an EC2 account

13. Click on “Sign up for Amazon EC2”

14. EC2 Account Signing up for EC2 automatically signs you up for Amazon Simple Storage Service, and Amazon Virtual Private Cloud. Requires credit card information. No charges until you start using the services. Amazon will email with Access Identifiers, and instructions for your first log in.

15. Click on “AWS Management Console”

16. Click the EC2 Tab

17. Launch an Instance

18. I recommend biolinux

19. Click “Select”

20. Pricing Amazon has a variety of VM sizes available – pricing is at: http://aws.amazon.com/ec2/pricing/ http://aws.amazon.com/ec2/pricing/ You are charged for CPU usage, for data storage, and for data transferred to or from Amazon. Charges continue until a VM is “Terminated”. You can set up a small test VM for free – select “Micro” for the size.

21. Kernel defaults are fine

22. Create a Key Pair

23. Create security group

24. Launch

25. Machine info

26. “Terminate” to end charges

27. ssh to the machine A window opens, telling you how to connect to your new VM, eg,: “ssh -i key_pair_name.pem root@ec2-76-202- 01-919.compute-1.amazonaws.com”root@ec2-76-202- 01-919.compute-1.amazonaws.com However, for biolinux, do: ssh –i key_pair_name.pem ubuntu@ec2-76-202- 01-919.compute-1.amazonaws.com

28. NX Use NX for the graphical display (built in to biolinux already). Open source, can be found at http://www.nomachine.com/http://www.nomachine.com/ Must ssh into VM FIRST, using the key pair. >adduser >groups >usermod -G,,ssh

29. Start NX

30. “Configure”

31. BioLinux over NX

32. Data Stored at Amazon There are large datasets stored at Amazon, available for use – free of charge (mostly). You are charged for any data you copy. http://aws.amazon.com/datasets to search through them.

33. http://aws.amazon.com/datasets

34. Datasets Human DNA sequences: 1000 Genomes Project (7,300 GB) Ensembl Annotated Human Genome - FASTA (115 GB) Ensembl Annotated Human Genome - MySQL (200 GB) GenBank (200 GB) Human Liver Cohort (Sage Bionetworks) (0.6 GB) Illumina - Jay Flatley's Human Genome Data Set. (350 GB) YRI Trio Data - complete genome sequence for three individuals (700 GB) Other (might include some human data): Ensembl - FASTA DB (100 GB) Influenza Virus (including Swine Flu) - from NCBI (1 GB) UniGene - from NCBI (10 GB) PubChem Library - from NCBI (230 GB)

35. Public Snapshots

37. Select “Volumes”

38. Create a Volume

39. Instance Information

40. Attach it to your Instance

41. Mount the Volume From your VM: >sudo mkfs –t ext3 /dev/sdf >sudo mkdir /mnt/datasets >sudo mount –t ext3 /dev/sdf /mnt/datasets 200GB of genbank data are now in /mnt/datasets