1. Reshma P Shetty, Drew Endy and Thomas F Knight Jr

2.  Background  Synthetic Biology  Terms  Method  Current Research  Discussion

3.  What is Synthetic Biology?  A Method  Milestones  Recombinant DNA  PCR(Polymerase Chain Reaction)  Automated Sequencing  Automated Construction  Standards  Abstractions -Write -------------------Read Last 30 years Synthetic Biology

4.  Systems  Devices  Parts  DNA

5.  Vector  Plasmid  Polymerase  Restriction enzyme  Ligase

6.  Goal  Standardize the BioBrick vectors’ assembly process  Maintain  Cloning site  Selection marker  High copy orgin  Terminators flanking the cloning site  Verification primer annealing sites  Process  Build new BioBrick vectors’ backbone  Standard assembly for new BioBrick

7.  Basic BioBrick

8.  Build new BioBrick vectors’ backbone

9.  Standard assembly for new BioBrick

10.  Check  Cloning site  Selection marker  High copy orgin  Terminators flanking the cloning site  Verification primer annealing sites

11.  More than 200 parts  Some Systems

12.  Wetware  Biological film  Digital counting machine  Interdisciplinary research  Computer aided design