1. D IVERSITY - BASED, MODEL - GUIDED CONSTRUCTION OF SYNTHETIC GENE NETWORKS WITH PREDICTED FUNCTIONS Tom Ellis, Xiao Wang & James J Collins 1 VC Lab, Dept. of Computer Science, NTHU, Taiwan

2. O UTLINE Introduction of System Biology Diversity-based, model-guided construction of synthetic gene networks with predicted functions Plug and play Experiment Result 2 VC Lab, Dept. of Computer Science, NTHU, Taiwan

3. S YSTEM B IOLOGY Developing and implementing computational- experimental methods to reverse engineer and analyze gene regulatory network Integrate—the soul Information—the basis Interference or control—the key Modeling & theorizing dependence –the feature Relationship to Synthetic Biology 3 VC Lab, Dept. of Computer Science, NTHU, Taiwan

4. P LUG AND P LAY Problem inadequately characterized time-consuming post hoc tweak Diversity-based, model-guided 4 VC Lab, Dept. of Computer Science, NTHU, Taiwan

5. E XPERIMENT (1/3) 5 VC Lab, Dept. of Computer Science, NTHU, Taiwan

6. E XPERIMENT (2/3) 6 VC Lab, Dept. of Computer Science, NTHU, Taiwan X Y Z NFL

7. E XPERIMENT (3/3) 7 VC Lab, Dept. of Computer Science, NTHU, Taiwan

8. R ESULT 8 VC Lab, Dept. of Computer Science, NTHU, Taiwan

9. D ISCUSSION Plug and play Same position, different promoters →many component in silico→front-end of gene network engineering 9 VC Lab, Dept. of Computer Science, NTHU, Taiwan

10. R EFERENCE Scott J Dixon, Brenda J Andrews, and Charles Boone, Exploring the conservation of synthetic lethal genetic interaction networks, Communicative & Integrative Biology 2, 78- 81(2009) Marcel Tigges, Tatiana T. Marquez-Lago, Jorg Stelling & Martin Fussenegger, A tunable synthetic mammalian oscillator, Nature 457, 309- 312(2009) 10 VC Lab, Dept. of Computer Science, NTHU, Taiwan