1. Vibrio Population Genetics Hopkins Microbiology Course 2011 Frank, Elsa, Bernal, Sasha, Tyler

2. Vibrio Population Genetics How are Vibrios and their genes distributed locally and globally?

3. Sampling Scheme for Multilocus Sequencing New Zealand: (2 coasts) x (2 beaches each) x (10 anemones each) x (5 Vibrio isolates each) California, 2007-2011: (Hopkins and Pt Lobos) x (8 anemones + 4 water each) x (5 Vibrio isolates each) Sequenced: recA, gyrB, mdh & ompK ATCGAT ATCGCG

4. Rarefaction 1%

5. California vs. New Zealand 1% Cutoff 5% Cutoff Total OTUs = 100 Total OTUs = 27 49 1 509 2 16 CANZ

6. California - Anemone vs. Water 1% Cutoff 5% Cutoff Total OTUs = 50 Total OTUs = 11 22 13 152 7 2 AnemoneWater

7. California - Hopkins vs. Point Lobos 1% Cutoff 5% Cutoff Hopkins Pt. Lobos Total OTUs = 50 Total OTUs = 11 30 11 94 6 1 Hopkins Pt. Lobos

8. California – 2008 to 2011 1% Cutoff 2010 1 0 0 0 4 3 2 15 0 2 7 0 1 0 8 2008 2009 5% Cutoff 2010 2011 0 0 0 0 0 0 0 0 4 1 2 2 0 2 0 Total OTUs = 43 Total OTUs = 11 2011 2008 2009

9. gyrB mdh recA California New Zealand

10. CaliforniaNew Zealand Concatenated Housekeeping by Location

11. Location Hopkins Point Lobos Year 2007 2008 2009 2010 2011 Association Anemone Water California Concatenated by:

12. Variable Selection ompK (2011 only) Hopkins Point Lobos

13. 2009 2007 2008 2011 2007 2009 2007 2011 2007  Water Anemone  NZ UNIFRAC Comparison P-value NZ vs CA0.01 Anemone vs Water0.28 (CA only) Between Years0.05-0.1 (CA only)

14. CALIFORNI A NEW ZEALAND Pop1Pop2Pop3Pop4Pop5 CA0.1300.4270.3820.0590.0002 NZ0.0120.0270.0100.4350.516 California vs. New Zealand

15. Locations within California Hopkins Pt. Lobos Pop 1Pop2Pop3 Hopkins0.280.170.57 Pt. Lobos0.520.090.39

16. 200 7 201 0 200 9 200 8 201 1 California Across Time

17. Point Lobos Hopkins Considering Allele Types recA gyrB mdh

18. Point Lobos Hopkins ompK recA gyrB mdh ompK across California & Allele Types

19. Conclusions & Future Directions Distinct local vs global patterns in Vibrio sequence types Investigate further: –Environmental data –Temporal data –Spatial distribution of hybridization –Competition experiments to determine local adaptation