1. RNA Polymerase binding *Constitutive or basal level transcription

2. Allosteric activation of RNA polymerase

3. Inhibition of transcription DNA-protein interactions

4. Negative Control of the lac Operon

5. Proposed CAP-cAMP Activation of lac Transcription

6. S.R. Eddy (2001) Noncoding RNA genes and the modern RNA world, Nature Reviews Genetics, 2:919-929 Three major classes of RNA: Transcription in Eukaryotes Type Encodes Copies/genome % Total RNA mRNA proteins Single or few 10% tRNA functional RNA Few 20% rRNA functional RNA Many 70% But there are more!!!

7. Epitope Tagging of RNA Polymerase II *Genetically “tag” Polymerase II subunit *Grow cells in radioactively labeled amino acids *Allow assembly of active polymerase *Add antibody that binds epitope *”Grab” antibody and what ever it is bound to *SDS electrophoresis

8. Transcription *RNA polymerase II

9. Transcription *RNA polymerase II