1. By: C.J. Beneduce Maria Guirguis Amanda Rode Mr. Normand Dubois

2. [Cyanomethyltriphenylphosphonium] + Bromide- (CMTPPB) Chemical Formula: C 20 H 17 BrNP Molecular Weight: 382.25 Melting Point: 279 C o

3. CMTPB and DNA Minor Groove Energy= -21.880026 kcal/mol Gradient= 0.099493 Major Groove Energy= -20.033628 kcal/mol Gradient= 0.089398 CMTPPB located is the minor groove of a 12 base pair DNA CMTPPB interacting with a 4 base pair DNA. Energy= -1.441035 kcal/mol Gradient=0.089282

4. Cary Spectrophotometer Data Tables DNA Temperature (C o )Absorbance 25 1.382 30 1.394 35 1.396 40 1.406 45 1.411 50 1.423 55 1.434 60 1.441 65 1.475 70 1.553 75 1.818 80 1.948 85 1.949 90 1.934 DNA and CMTPPB Temperature (C o) Absorbance 25 1.549 30 1.559 35 1.56 40 1.561 45 1.563 50 1.561 55 1.565 60 1.573 65 1.588 70 1.693 75 2.186 80 2.729 85 2.947 90 2.994

5. Cary Spectrophotometer Graphs

7. Electrophoresis From left to right: linearized DNA(E), linearized DNA and CMTPPB(EB), plasmid DNA(X), plasmid DNA and CMTPPB(XB), and ladder (L). Trail 1 E- Band #1= 25cm EB- Band #1= 25cm X- Band #1= 22cm Band #2= 24cm Band #3= 28cm XB- Band #1= 21cm Band #2= 29cm L- Band #1= 16cm Band #2= 20cm Band #3= 23cm Band #4= 28cm

8. Discussion The results indicated that CMTPPB may have bound with the DNA. The evidence for this was: A negative energy on HyperChem On the Cary Spectrophotometer, there was a shift in the melting curve of the DNA and CMTPPB sample, compared to the native DNA sample For future investigations: More trials Different concentrations of the compound of interest