1. By: Destiny Sanchez Efren Miranda Mirna Rocha

2.  A Quantitative Trait is a trait of a gene that can be described with numbers.  QTL (Quantitative Trait Loci) are the locations on chromosomes where the genes that influence quantitative traits reside. (Robert J. Brooker) QTL Phenotype

3.  HDL Cholesterol (High Density Lipoproteins)  LDL Cholesterol (Low Density Lipoproteins)  Atherosclerosis

4.  Heart attacks and Stroke are a result of atherosclerosis  A build up of fat around arterial walls will eventually lead to complete arterial blockage  Could result in death

5.  To perform QTL analysis on inbred mice in order to determine the effects of a high fat diet on HDL concentrations and Atherosclerosis susceptibility.

6. C57BL/6J (B6) X 129S1/SvImJ(129) B6x129 (F1) F2 Progeny

7.  Fed high diet to 294 (F2) female mice for 14 weeks  Measured plasma HDL concentrations  Measured size of aortic fatty-streak lesions  Performed QTL analysis

8. MicenPlasma HDLAortic Lesions B61041±2951±459 12910106±40 F1884±30 F229481±1228±44

11.  B6 mice displayed low HDL and High Atherosclerosis susceptibility  F2 intercross - 6 Loci for HDL were identified and 5 loci for heart disease susceptibility  129 mice had high HDL levels and Atherosclerosis resistance

12.  A high fat diet plays a large role in the damaging of the aortic system leading to heart disease.  The QTL for traits are located on the same regions of the chromosomes in mice and humans.

13. High fiber diet Vegetables Low Fat Lean Protein

14.  Brooker, Robert J.. Genetics Analysis and Principles. New York, NY: McGraw-Hill, 2009.  http://beckerinfo.net/bioinformatics/?p=111 8

15. Dr. Randy Von Smith Tobias Beckwith Dr. Brook Milligan RISE Program